Sitotoksisitas resin akrilik hybrid setelah penambahan glass fiber dengan metode berbeda (Cytotoxicity of the hybrid acrylic resin after glass fiber reinforcement with difference method)

• Main Authors: Intan Nirwana, R. Helal Soekartono
• Format: Article
• Language: English
• Published: Universitas Airlangga 2005-06-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: hybrid acrylic resins; cytotoxicity; glass fiber
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/1139

<span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>Glass fiber reinforcement of the hybrid acrylic resin with difference method can enhance residual monomer content of the </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>material; it can cause cytotoxic effect on fibroblast cells. The purpose of this study was to know the cytotoxicity of hybrid acrylic </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>resins after glass fiber reinforcement with difference method on the cultured fibroblasts. The squared specimens of 10 mm in length, </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>10 mm in width and 1.5 mm in thickness were cured for 20 minutes at 100° C. The fibroblast cells were grown in Eagle's Minimum </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>Essential Medium to be 2 </em><span style="font-family: Arial-ItalicMT; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>× </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>10</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #000000; font-style: normal; font-variant: normal;"><em>5 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>cells/ml, then the cells were added to the samples in the plates and incubated at 37° C. After 48 hours, </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>the cytotoxic effect was determined by direct cell number count using microscope and a hemocytometer. The statistical analyses </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>using one way ANOVA and LSD test showed that there were significant difference in cell viability (p &lt; 0.05) among the groups. The </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>means percentage of cell viability were 90.00%, 99.,11%, 98.66%, it could be concluded that glass fiber reinforcement into hybrid </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>acrylic resin with either first method or second method was not toxic.</em></span></span></span></span></span></span></span></span></span></span></span></span></span>