Effect of Xenon Treatment on Gene Expression in Brain Tissue after Traumatic Brain Injury in Rats

• Main Authors: Anton D. Filev, Denis N. Silachev, Ivan A. Ryzhkov, Konstantin N. Lapin, Anastasiya S. Babkina, Oleg A. Grebenchikov, Vladimir M. Pisarev
• Format: Article
• Language: English
• Published: MDPI AG 2021-07-01
• Series: Brain Sciences
• Subjects: TBI; xenon; neuroinflammation; Nanostring; rat; gene expression
• Online Access: https://www.mdpi.com/2076-3425/11/7/889

The overactivation of inflammatory pathways and/or a deficiency of neuroplasticity may result in the delayed recovery of neural function in traumatic brain injury (TBI). A promising approach to protecting the brain tissue in TBI is xenon (Xe) treatment. However, xenon’s mechanisms of action remain poorly clarified. In this study, the early-onset expression of 91 target genes was investigated in the damaged and in the contralateral brain areas (sensorimotor cortex region) 6 and 24 h after injury in a TBI rat model. The expression of genes involved in inflammation, oxidation, antioxidation, neurogenesis and neuroplasticity, apoptosis, DNA repair, autophagy, and mitophagy was assessed. The animals inhaled a gas mixture containing xenon and oxygen (ϕXe = 70%; ϕO₂ 25–30% 60 min) 15–30 min after TBI. The data showed that, in the contralateral area, xenon treatment induced the expression of stress genes (<i>Irf1</i>, <i>Hmox1</i>, <i>S100A8</i>, and <i>S100A9</i>). In the damaged area, a trend towards lower expression of the inflammatory gene <i>Irf1</i> was observed. Thus, our results suggest that xenon exerts a mild stressor effect in healthy brain tissue and has a tendency to decrease the inflammation following damage, which might contribute to reducing the damage and activating the early compensatory processes in the brain post-TBI.