Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model.
Mitochondrial electron transport chain (ETC) defects are observed in Parkinson's disease (PD) patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that e...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3823844?pdf=render |
id |
doaj-6d0b93c3ff114760812c7c0d1c08a002 |
---|---|
record_format |
Article |
spelling |
doaj-6d0b93c3ff114760812c7c0d1c08a0022020-11-25T01:34:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7856210.1371/journal.pone.0078562Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model.Melissa VosBlaise LovisaAnn GeensVanessa A MoraisGeorges WagnièresHubert van den BerghAlec GinggenBart De StrooperYanik TardyPatrik VerstrekenMitochondrial electron transport chain (ETC) defects are observed in Parkinson's disease (PD) patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that effectively penetrates tissues rescues pink1 mutants. We show that irradiating isolated fly or mouse mitochondria with 808 nm light that is absorbed by ETC-Complex IV acutely improves Complex IV-dependent oxygen consumption and ATP production, a feature that is wavelength-specific. Irradiating Drosophila pink1 mutants using a single dose of 808 nm light results in a rescue of major systemic and mitochondrial defects. Time-course experiments indicate mitochondrial membrane potential defects are rescued prior to mitochondrial morphological defects, also in dopaminergic neurons, suggesting mitochondrial functional defects precede mitochondrial swelling. Thus, our data indicate that improvement of mitochondrial function using infrared light stimulation is a viable strategy to alleviate pink1-related defects.http://europepmc.org/articles/PMC3823844?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Melissa Vos Blaise Lovisa Ann Geens Vanessa A Morais Georges Wagnières Hubert van den Bergh Alec Ginggen Bart De Strooper Yanik Tardy Patrik Verstreken |
spellingShingle |
Melissa Vos Blaise Lovisa Ann Geens Vanessa A Morais Georges Wagnières Hubert van den Bergh Alec Ginggen Bart De Strooper Yanik Tardy Patrik Verstreken Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. PLoS ONE |
author_facet |
Melissa Vos Blaise Lovisa Ann Geens Vanessa A Morais Georges Wagnières Hubert van den Bergh Alec Ginggen Bart De Strooper Yanik Tardy Patrik Verstreken |
author_sort |
Melissa Vos |
title |
Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. |
title_short |
Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. |
title_full |
Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. |
title_fullStr |
Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. |
title_full_unstemmed |
Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model. |
title_sort |
near-infrared 808 nm light boosts complex iv-dependent respiration and rescues a parkinson-related pink1 model. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Mitochondrial electron transport chain (ETC) defects are observed in Parkinson's disease (PD) patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that effectively penetrates tissues rescues pink1 mutants. We show that irradiating isolated fly or mouse mitochondria with 808 nm light that is absorbed by ETC-Complex IV acutely improves Complex IV-dependent oxygen consumption and ATP production, a feature that is wavelength-specific. Irradiating Drosophila pink1 mutants using a single dose of 808 nm light results in a rescue of major systemic and mitochondrial defects. Time-course experiments indicate mitochondrial membrane potential defects are rescued prior to mitochondrial morphological defects, also in dopaminergic neurons, suggesting mitochondrial functional defects precede mitochondrial swelling. Thus, our data indicate that improvement of mitochondrial function using infrared light stimulation is a viable strategy to alleviate pink1-related defects. |
url |
http://europepmc.org/articles/PMC3823844?pdf=render |
work_keys_str_mv |
AT melissavos nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT blaiselovisa nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT anngeens nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT vanessaamorais nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT georgeswagnieres nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT hubertvandenbergh nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT alecginggen nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT bartdestrooper nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT yaniktardy nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model AT patrikverstreken nearinfrared808nmlightboostscomplexivdependentrespirationandrescuesaparkinsonrelatedpink1model |
_version_ |
1725069860869767168 |