Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry
High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high f...
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MDPI AG
2021-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/3/345 |
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doaj-6d0761c6e94f47c58fe525d295e58ed62021-03-24T00:06:10ZengMDPI AGMicromachines2072-666X2021-03-011234534510.3390/mi12030345Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow CytometryKaiser Pärnamets0Tamas Pardy1Ants Koel2Toomas Rang3Ott Scheler4Yannick Le Moullec5Fariha Afrin6Thomas Johann Seebeck Department of Electronics, Tallinn University of Technology, 19086 Tallinn, EstoniaDepartment of Chemistry and Biotechnology, Tallinn University of Technology, 19086 Tallinn, EstoniaThomas Johann Seebeck Department of Electronics, Tallinn University of Technology, 19086 Tallinn, EstoniaThomas Johann Seebeck Department of Electronics, Tallinn University of Technology, 19086 Tallinn, EstoniaDepartment of Chemistry and Biotechnology, Tallinn University of Technology, 19086 Tallinn, EstoniaThomas Johann Seebeck Department of Electronics, Tallinn University of Technology, 19086 Tallinn, EstoniaThomas Johann Seebeck Department of Electronics, Tallinn University of Technology, 19086 Tallinn, EstoniaHigh-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high flow rates, the droplets allow massive parallelization, further increasing the throughput of droplets. However, this novel methodology poses unique challenges related to commonly used fluorometry and fluorescent microscopy techniques. We review the optical sensor technology and light sources applicable to DMFC, as well as analyze the challenges and advantages of each option, primarily focusing on electronics. An analysis of low-cost and/or sufficiently compact systems that can be incorporated into portable devices is also presented.https://www.mdpi.com/2072-666X/12/3/345droplet microfluidicsoptical sensorslight sourcesmicroflow cytometry |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Kaiser Pärnamets Tamas Pardy Ants Koel Toomas Rang Ott Scheler Yannick Le Moullec Fariha Afrin |
| spellingShingle |
Kaiser Pärnamets Tamas Pardy Ants Koel Toomas Rang Ott Scheler Yannick Le Moullec Fariha Afrin Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry Micromachines droplet microfluidics optical sensors light sources microflow cytometry |
| author_facet |
Kaiser Pärnamets Tamas Pardy Ants Koel Toomas Rang Ott Scheler Yannick Le Moullec Fariha Afrin |
| author_sort |
Kaiser Pärnamets |
| title |
Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
| title_short |
Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
| title_full |
Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
| title_fullStr |
Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
| title_full_unstemmed |
Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
| title_sort |
optical detection methods for high-throughput fluorescent droplet microflow cytometry |
| publisher |
MDPI AG |
| series |
Micromachines |
| issn |
2072-666X |
| publishDate |
2021-03-01 |
| description |
High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high flow rates, the droplets allow massive parallelization, further increasing the throughput of droplets. However, this novel methodology poses unique challenges related to commonly used fluorometry and fluorescent microscopy techniques. We review the optical sensor technology and light sources applicable to DMFC, as well as analyze the challenges and advantages of each option, primarily focusing on electronics. An analysis of low-cost and/or sufficiently compact systems that can be incorporated into portable devices is also presented. |
| topic |
droplet microfluidics optical sensors light sources microflow cytometry |
| url |
https://www.mdpi.com/2072-666X/12/3/345 |
| work_keys_str_mv |
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