A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System
The “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabd...
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Neural Circuits |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncir.2018.00094/full |
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doaj-6d03b29f89cc46d4a2783e77dccaba4e2020-11-24T21:54:53ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102018-11-011210.3389/fncir.2018.00094419735A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous SystemBen Mulcahy0Daniel Witvliet1Daniel Witvliet2Douglas Holmyard3Douglas Holmyard4James Mitchell5Andrew D. Chisholm6Yaron Meirovitch7Aravinthan D. T. Samuel8Mei Zhen9Mei Zhen10Mei Zhen11Mei Zhen12Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, ON, CanadaDepartment of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, CanadaNanoscale Biomedical Imaging Facility, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, CanadaCenter for Brain Science, Department of Physics, Harvard University, Cambridge, MA, United StatesSection of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA, United StatesComputer Science and Artificial Intelligence Laboratory, MIT, Cambridge, MA, United StatesCenter for Brain Science, Department of Physics, Harvard University, Cambridge, MA, United StatesLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, ON, CanadaDepartment of Physiology, University of Toronto, Toronto, ON, CanadaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, CanadaThe “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabditis elegans. Recent advances in vEM allow mapping new C. elegans connectomes with increased throughput, and reduced subjectivity. Current vEM studies aim to not only fill the remaining gaps in the original connectome, but also address fundamental questions including how the connectome changes during development, the nature of individuality, sexual dimorphism, and how genetic and environmental factors regulate connectivity. Here we describe our current vEM pipeline and projected improvements for the study of the C. elegans nervous system and beyond.https://www.frontiersin.org/article/10.3389/fncir.2018.00094/fullC. elegansvolume electron microscopyconnectomenervous systemhigh-pressure freezing |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Ben Mulcahy Daniel Witvliet Daniel Witvliet Douglas Holmyard Douglas Holmyard James Mitchell Andrew D. Chisholm Yaron Meirovitch Aravinthan D. T. Samuel Mei Zhen Mei Zhen Mei Zhen Mei Zhen |
| spellingShingle |
Ben Mulcahy Daniel Witvliet Daniel Witvliet Douglas Holmyard Douglas Holmyard James Mitchell Andrew D. Chisholm Yaron Meirovitch Aravinthan D. T. Samuel Mei Zhen Mei Zhen Mei Zhen Mei Zhen A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System Frontiers in Neural Circuits C. elegans volume electron microscopy connectome nervous system high-pressure freezing |
| author_facet |
Ben Mulcahy Daniel Witvliet Daniel Witvliet Douglas Holmyard Douglas Holmyard James Mitchell Andrew D. Chisholm Yaron Meirovitch Aravinthan D. T. Samuel Mei Zhen Mei Zhen Mei Zhen Mei Zhen |
| author_sort |
Ben Mulcahy |
| title |
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System |
| title_short |
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System |
| title_full |
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System |
| title_fullStr |
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System |
| title_full_unstemmed |
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System |
| title_sort |
pipeline for volume electron microscopy of the caenorhabditis elegans nervous system |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Neural Circuits |
| issn |
1662-5110 |
| publishDate |
2018-11-01 |
| description |
The “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabditis elegans. Recent advances in vEM allow mapping new C. elegans connectomes with increased throughput, and reduced subjectivity. Current vEM studies aim to not only fill the remaining gaps in the original connectome, but also address fundamental questions including how the connectome changes during development, the nature of individuality, sexual dimorphism, and how genetic and environmental factors regulate connectivity. Here we describe our current vEM pipeline and projected improvements for the study of the C. elegans nervous system and beyond. |
| topic |
C. elegans volume electron microscopy connectome nervous system high-pressure freezing |
| url |
https://www.frontiersin.org/article/10.3389/fncir.2018.00094/full |
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