INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO

Objective: to evaluate the effect of NSAIDs eye drops with different preservatives on the viability of epithelial cells of human eyes in vitro. Materials and methods. The object of the study was four of the drug Broxinac®, Acular LS®, Diclo-F ®, Indocollyre®. As test-systems were used permanent cell...

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Main Authors: O. I. Aleksandrova, I. N. Okolov, Yu. I. Khorolskaya, I. E. Panovа, M. I. Blinova
Format: Article
Language:Russian
Published: Ophthalmology Publishing Group 2017-10-01
Series:Oftalʹmologiâ
Subjects:
Online Access:https://www.ophthalmojournal.com/opht/article/view/398
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spelling doaj-6d0297f1d1484797982bcdcaeb86a2b82021-07-29T08:55:25ZrusOphthalmology Publishing GroupOftalʹmologiâ 1816-50952017-10-0114325125910.18008/1816-5095-2017-3-251-259356INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITROO. I. Aleksandrova0I. N. Okolov1Yu. I. Khorolskaya2I. E. Panovа3M. I. Blinova4Institute of Cytology of the Russian Academy of Science, Saint PetersburgSv. Fyodorov Eye Microsurgery Federal State InstitutionInstitute of Cytology of the Russian Academy of Science, Saint PetersburgSv. Fyodorov Eye Microsurgery Federal State InstitutionInstitute of Cytology of the Russian Academy of Science, Saint PetersburgObjective: to evaluate the effect of NSAIDs eye drops with different preservatives on the viability of epithelial cells of human eyes in vitro. Materials and methods. The object of the study was four of the drug Broxinac®, Acular LS®, Diclo-F ®, Indocollyre®. As test-systems were used permanent cell lines of transformed conjunctival (Chang Conjunctiva, Clone 1-5c-4) and corneal (HCEC). The cytotoxicity of NSAID drops were assessed by the morphology and functional cells activity by the methods of phase contrast microscopy (PCM), MTT test and cell analysis in real time using the xCELLigence system. Results. It was found with MTT-test that cell lines HCEC are more sensitive to the cytotoxic action of the studied drugs than cell lines of Chang Conjunctiva, Clone 1-5C-4. The metabolic activity of conjunctiva cells in the presence of drugs, Acular LS® and Broxinac® was three times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® — 20 times lower vs. the control. The metabolic activity of the cornea cells e in the presence of drugs, Acular LS® and Broxinac® was four times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® viable cells were not identified. Drugs Indocollyre® and Diclo-F® has had a very high toxic effect on both cell types. The results of cell analysis xCELLIgence for both types of cells are consistent with the results of MTT test and in vivo observation of cells. Conclusion. Based on these data, there is the following gradation of NSAID eye drop’s cytotoxic potential: (reduce toxicity): Indocollyre® = Diclo-F® > Acular LS® = Broxinac®.https://www.ophthalmojournal.com/opht/article/view/398cell culturesxcelligenceconjunctivapreservativesnsaidcorneacytotoxicityocular surface epithelium
collection DOAJ
language Russian
format Article
sources DOAJ
author O. I. Aleksandrova
I. N. Okolov
Yu. I. Khorolskaya
I. E. Panovа
M. I. Blinova
spellingShingle O. I. Aleksandrova
I. N. Okolov
Yu. I. Khorolskaya
I. E. Panovа
M. I. Blinova
INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
Oftalʹmologiâ
cell cultures
xcelligence
conjunctiva
preservatives
nsaid
cornea
cytotoxicity
ocular surface epithelium
author_facet O. I. Aleksandrova
I. N. Okolov
Yu. I. Khorolskaya
I. E. Panovа
M. I. Blinova
author_sort O. I. Aleksandrova
title INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
title_short INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
title_full INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
title_fullStr INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
title_full_unstemmed INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO
title_sort influence of non-steroidal anti-inflammatory eye drops on the epithelium cells of the cornea and conjunctiva in vitro
publisher Ophthalmology Publishing Group
series Oftalʹmologiâ
issn 1816-5095
publishDate 2017-10-01
description Objective: to evaluate the effect of NSAIDs eye drops with different preservatives on the viability of epithelial cells of human eyes in vitro. Materials and methods. The object of the study was four of the drug Broxinac®, Acular LS®, Diclo-F ®, Indocollyre®. As test-systems were used permanent cell lines of transformed conjunctival (Chang Conjunctiva, Clone 1-5c-4) and corneal (HCEC). The cytotoxicity of NSAID drops were assessed by the morphology and functional cells activity by the methods of phase contrast microscopy (PCM), MTT test and cell analysis in real time using the xCELLigence system. Results. It was found with MTT-test that cell lines HCEC are more sensitive to the cytotoxic action of the studied drugs than cell lines of Chang Conjunctiva, Clone 1-5C-4. The metabolic activity of conjunctiva cells in the presence of drugs, Acular LS® and Broxinac® was three times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® — 20 times lower vs. the control. The metabolic activity of the cornea cells e in the presence of drugs, Acular LS® and Broxinac® was four times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® viable cells were not identified. Drugs Indocollyre® and Diclo-F® has had a very high toxic effect on both cell types. The results of cell analysis xCELLIgence for both types of cells are consistent with the results of MTT test and in vivo observation of cells. Conclusion. Based on these data, there is the following gradation of NSAID eye drop’s cytotoxic potential: (reduce toxicity): Indocollyre® = Diclo-F® > Acular LS® = Broxinac®.
topic cell cultures
xcelligence
conjunctiva
preservatives
nsaid
cornea
cytotoxicity
ocular surface epithelium
url https://www.ophthalmojournal.com/opht/article/view/398
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