Genome Sequence Analysis of <i>Auricularia heimuer</i> Combined with Genetic Linkage Map

• Main Authors: Ming Fang, Xiaoe Wang, Ying Chen, Peng Wang, Lixin Lu, Jia Lu, Fangjie Yao, Youmin Zhang
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Journal of Fungi
• Subjects: <i>auricularia heimuer</i>; alcohol dehydrogenase; cazymes; genome; linkage map; mating-type; phylogeny
• Online Access: https://www.mdpi.com/2309-608X/6/1/37

<i>Auricularia heimuer</i> is one of the most popular edible fungi in China. In this study, the whole genome of <i>A. heimuer</i> was sequenced on the Illumina HiSeq X system and compared with other mushrooms genomes. As a wood-rotting fungus, a total of 509 carbohydrate-active enzymes (CAZymes) were annotated in order to explore its potential capabilities on wood degradation. The glycoside hydrolases (GH) family genes in the <i>A. heimuer</i> genome were more abundant than the genes in the other 11 mushrooms genomes. The <i>A. heimuer</i> genome contained 102 genes encoding class III, IV, and V ethanol dehydrogenases. Evolutionary analysis based on 562 orthologous single-copy genes from 15 mushrooms showed that <i>Auricularia</i> formed an early independent branch of Agaricomycetes. The mating-type locus of <i>A. heimuer</i> was located on linkage group 8 by genetic linkage analysis. By combining the genome sequence analysis with the genetic linkage map, the mating-type locus of <i>A. heimuer</i> was located on scaffold45 and consisted of two subunits, &#945; and &#946;. Each subunit consisted of a pair of homeodomain mating-type protein genes <i>HD1</i> and <i>HD2</i>. The mapping revealed conserved synteny at the whole mating-type loci and mirror symmetry relations near the &#946; subunit between <i>A. heimuer</i> and <i>Exidia glandulosa</i>. This study proposed the potential for the bioethanol production by consolidated bioprocessing of <i>A. heimuer</i>. It will promote understanding of the lignocellulose degradation system and facilitate more efficient conversion of the agricultural wastes used for mushroom cultivation. It also will advance the research on the fruiting body development and evolution of <i>A. heimuer</i>.