Multilocus DNA-fingerprinting using oligonucleotide probes (GATA)4 and (GGAT)4 in rainbow trout (Oncorhynchus mykiss)
Oligonucleotide probes (GATA)4 and (GGAT)4 for minisatellite loci produced DNA fingerprinting patterns which are unique in individual rainbow trout. These patterns can be used to monitor the efficiency of manipulation of production characters in gynogenetic individuals. The (GATA)4 probe produced hi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
1998-06-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200011 |
| Summary: | Oligonucleotide probes (GATA)4 and (GGAT)4 for minisatellite loci produced DNA fingerprinting patterns which are unique in individual rainbow trout. These patterns can be used to monitor the efficiency of manipulation of production characters in gynogenetic individuals. The (GATA)4 probe produced highly individual patterns that differentiated individuals among gynogenetic offspring, and could be easily scored.<br>Probes de oligonucleotídeos (GATA)4 e (GGAT)4 para locos mini-satélites produziram padrões de "fingerprinting" de DNA que são específicos para cada espécimen de truta do arco-íris. Esses padrões podem ser usados para monitorar a eficácia da manipulação de caracteres de produção em indivíduos ginogenéticos. O probe (GATA)4 produziu padrões altamente individuais que diferenciaram os indivíduos entre proles ginogenéticas e puderam ser facilmente quantificados. |
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| ISSN: | 1415-4757 1678-4685 |