Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production

<p>Abstract</p> <p>Background</p> <p>Fermentations using <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST), and <it>Zymomonas mobilis </it>AX101 are compared side-by-side on corn steep liquor (CSL) media a...

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Main Authors: Dale Bruce E, Balan Venkatesh, Gunawan Christa, Lau Ming W
Format: Article
Language:English
Published: BMC 2010-05-01
Series:Biotechnology for Biofuels
Online Access:http://www.biotechnologyforbiofuels.com/content/3/1/11
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spelling doaj-6cc25f2a90a54600bba057fc9b3a48392020-11-25T00:18:45ZengBMCBiotechnology for Biofuels1754-68342010-05-01311110.1186/1754-6834-3-11Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol productionDale Bruce EBalan VenkateshGunawan ChristaLau Ming W<p>Abstract</p> <p>Background</p> <p>Fermentations using <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST), and <it>Zymomonas mobilis </it>AX101 are compared side-by-side on corn steep liquor (CSL) media and the water extract and enzymatic hydrolysate from ammonia fiber expansion (AFEX)-pretreated corn stover.</p> <p>Results</p> <p>The three ethanologens are able produce ethanol from a CSL-supplemented co-fermentation at a metabolic yield, final concentration and rate greater than 0.42 g/g consumed sugars, 40 g/L and 0.7 g/L/h (0-48 h), respectively. Xylose-only fermentation of the tested ethanologenic bacteria are five to eight times faster than 424A(LNH-ST) in the CSL fermentation.</p> <p>All tested strains grow and co-ferment sugars at 15% w/v solids loading equivalent of ammonia fiber explosion (AFEX)-pretreated corn stover water extract. However, both KO11 and 424A(LNH-ST) exhibit higher growth robustness than AX101. In 18% w/w solids loading lignocellulosic hydrolysate from AFEX pretreatment, complete glucose fermentations can be achieved at a rate greater than 0.77 g/L/h. In contrast to results from fermentation in CSL, <it>S. cerevisiae </it>424A(LNH-ST) consumed xylose at the greatest extent and rate in the hydrolysate compared to the bacteria tested.</p> <p>Conclusions</p> <p>Our results confirm that glucose fermentations among the tested strains are effective even at high solids loading (18% by weight). However, xylose consumption in the lignocellulosic hydrolysate is the major bottleneck affecting overall yield, titer or rate of the process. In comparison, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) is the most relevant strains for industrial production for its ability to ferment both glucose and xylose from undetoxified and unsupplemented hydrolysate from AFEX-pretreated corn stover at high yield.</p> http://www.biotechnologyforbiofuels.com/content/3/1/11
collection DOAJ
language English
format Article
sources DOAJ
author Dale Bruce E
Balan Venkatesh
Gunawan Christa
Lau Ming W
spellingShingle Dale Bruce E
Balan Venkatesh
Gunawan Christa
Lau Ming W
Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
Biotechnology for Biofuels
author_facet Dale Bruce E
Balan Venkatesh
Gunawan Christa
Lau Ming W
author_sort Dale Bruce E
title Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
title_short Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
title_full Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
title_fullStr Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
title_full_unstemmed Comparing the fermentation performance of <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) and <it>Zymomonas mobilis </it>AX101 for cellulosic ethanol production
title_sort comparing the fermentation performance of <it>escherichia coli </it>ko11, <it>saccharomyces cerevisiae </it>424a(lnh-st) and <it>zymomonas mobilis </it>ax101 for cellulosic ethanol production
publisher BMC
series Biotechnology for Biofuels
issn 1754-6834
publishDate 2010-05-01
description <p>Abstract</p> <p>Background</p> <p>Fermentations using <it>Escherichia coli </it>KO11, <it>Saccharomyces cerevisiae </it>424A(LNH-ST), and <it>Zymomonas mobilis </it>AX101 are compared side-by-side on corn steep liquor (CSL) media and the water extract and enzymatic hydrolysate from ammonia fiber expansion (AFEX)-pretreated corn stover.</p> <p>Results</p> <p>The three ethanologens are able produce ethanol from a CSL-supplemented co-fermentation at a metabolic yield, final concentration and rate greater than 0.42 g/g consumed sugars, 40 g/L and 0.7 g/L/h (0-48 h), respectively. Xylose-only fermentation of the tested ethanologenic bacteria are five to eight times faster than 424A(LNH-ST) in the CSL fermentation.</p> <p>All tested strains grow and co-ferment sugars at 15% w/v solids loading equivalent of ammonia fiber explosion (AFEX)-pretreated corn stover water extract. However, both KO11 and 424A(LNH-ST) exhibit higher growth robustness than AX101. In 18% w/w solids loading lignocellulosic hydrolysate from AFEX pretreatment, complete glucose fermentations can be achieved at a rate greater than 0.77 g/L/h. In contrast to results from fermentation in CSL, <it>S. cerevisiae </it>424A(LNH-ST) consumed xylose at the greatest extent and rate in the hydrolysate compared to the bacteria tested.</p> <p>Conclusions</p> <p>Our results confirm that glucose fermentations among the tested strains are effective even at high solids loading (18% by weight). However, xylose consumption in the lignocellulosic hydrolysate is the major bottleneck affecting overall yield, titer or rate of the process. In comparison, <it>Saccharomyces cerevisiae </it>424A(LNH-ST) is the most relevant strains for industrial production for its ability to ferment both glucose and xylose from undetoxified and unsupplemented hydrolysate from AFEX-pretreated corn stover at high yield.</p>
url http://www.biotechnologyforbiofuels.com/content/3/1/11
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