Effects of Antimicrobial Photodynamic Therapy on Organic Solution and Root Surface In Vitro

• Main Authors: Yuji Matsushima, Akihiro Yashima, Meri Fukaya, Satoshi Shirakawa, Tomoko Ohshima, Tomomi Kawai, Takatoshi Nagano, Kazuhiro Gomi
• Format: Article
• Language: English
• Published: MDPI AG 2021-01-01
• Series: Antibiotics
• Subjects: photodynamic therapy; antibacterial therapy; bacterial flora; dental treatment; lipopolysaccharide; organic resolution
• Online Access: https://www.mdpi.com/2079-6382/10/2/101

Antimicrobial photodynamic therapy (a-PDT) is attracting attention as a new form of dental treatment. While it is primarily applied to produce an antibacterial effect, it decreases lipopolysaccharide (LPS) and protease activity. Here, we evaluated differences in the antibacterial activity of a-PDT on three types of bacteria and the effects on the organic substances (i.e., albumin and LPS). Furthermore, we investigated the effects of a-PDT on root surfaces. A FotoSan630^® and toluidine blue were used to perform a-PDT in this study. We measured its antimicrobial activity against <i>Porphyromonas gingivalis</i>, <i>Streptococcus mutans</i>, and <i>Enterococcus faecalis</i>. Antimicrobial testing revealed strong antimicrobial action and <i>P. gingivalis</i>, <i>E. faecalis</i>, and <i>S. mutans</i> were almost undetectable after 50, 120, and 100 s, respectively. In organic resolution tests, albumin was significantly decreased from 1 min after a-PDT application onward, while LPS significantly decreased at 5 min after the application. The root surfaces after a-PDT were confirmed to be cleaner than the controls without suffering any damage. Depending on the bacterial species, a-PDT exhibited antimicrobial activity against various types of bacteria and sensitivity differed. Moreover, we reported that a-PDT resolves protein and LPS, enabling the formation of a healthy root surface without any damage.