Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model

Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model

The objective of this study was to investigate the effects of iron dextran on lipid metabolism and to determine the involvement of oxidative stress. Fischer rats were divided into two groups: the standard group (S), which was fed the AIN-93M diet, and the standard plus iron group (SI), which was fed...

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Main Authors: Maísa Silva, Joyce Ferreira da Costa Guerra, Ana Flávia Santos Sampaio, Wanderson Geraldo de Lima, Marcelo Eustáquio Silva, Maria Lucia Pedrosa
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2015/272617
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spelling doaj-6c84486086d345c6ae1b3f87575901fd2020-11-25T00:34:54ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/272617272617Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine ModelMaísa Silva0Joyce Ferreira da Costa Guerra1Ana Flávia Santos Sampaio2Wanderson Geraldo de Lima3Marcelo Eustáquio Silva4Maria Lucia Pedrosa5Basic Department-Health Area, Federal University of Juiz de Fora, Campus Universitário s/n, 36036-900 Juiz de Fora, MG, BrazilResearch Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, BrazilProgram of Health and Nutrition, Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, BrazilResearch Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, BrazilResearch Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, BrazilResearch Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, BrazilThe objective of this study was to investigate the effects of iron dextran on lipid metabolism and to determine the involvement of oxidative stress. Fischer rats were divided into two groups: the standard group (S), which was fed the AIN-93M diet, and the standard plus iron group (SI), which was fed the same diet but also received iron dextran injections. Serum cholesterol and triacylglycerol levels were higher in the SI group than in the S group. Iron dextran was associated with decreased mRNA levels of pparα, and its downstream gene cpt1a, which is involved in lipid oxidation. Iron dextran also increased mRNA levels of apoB-100, MTP, and L-FABP indicating alterations in lipid secretion. Carbonyl protein and TBARS were consistently higher in the liver of the iron-treated rats. Moreover, a significant positive correlation was found between oxidative stress products, lfabp expression, and iron stores. In addition, a negative correlation was found between pparα expression, TBARS, carbonyl protein, and iron stores. In conclusion, our results suggest that the increase observed in the transport of lipids in the bloodstream and the decreased fatty acid oxidation in rats, which was promoted by iron dextran, might be attributed to increased oxidative stress.http://dx.doi.org/10.1155/2015/272617
collection DOAJ
language English
format Article
sources DOAJ
author Maísa Silva
Joyce Ferreira da Costa Guerra
Ana Flávia Santos Sampaio
Wanderson Geraldo de Lima
Marcelo Eustáquio Silva
Maria Lucia Pedrosa
spellingShingle Maísa Silva
Joyce Ferreira da Costa Guerra
Ana Flávia Santos Sampaio
Wanderson Geraldo de Lima
Marcelo Eustáquio Silva
Maria Lucia Pedrosa
Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
BioMed Research International
author_facet Maísa Silva
Joyce Ferreira da Costa Guerra
Ana Flávia Santos Sampaio
Wanderson Geraldo de Lima
Marcelo Eustáquio Silva
Maria Lucia Pedrosa
author_sort Maísa Silva
title Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
title_short Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
title_full Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
title_fullStr Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
title_full_unstemmed Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model
title_sort iron dextran increases hepatic oxidative stress and alters expression of genes related to lipid metabolism contributing to hyperlipidaemia in murine model
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2015-01-01
description The objective of this study was to investigate the effects of iron dextran on lipid metabolism and to determine the involvement of oxidative stress. Fischer rats were divided into two groups: the standard group (S), which was fed the AIN-93M diet, and the standard plus iron group (SI), which was fed the same diet but also received iron dextran injections. Serum cholesterol and triacylglycerol levels were higher in the SI group than in the S group. Iron dextran was associated with decreased mRNA levels of pparα, and its downstream gene cpt1a, which is involved in lipid oxidation. Iron dextran also increased mRNA levels of apoB-100, MTP, and L-FABP indicating alterations in lipid secretion. Carbonyl protein and TBARS were consistently higher in the liver of the iron-treated rats. Moreover, a significant positive correlation was found between oxidative stress products, lfabp expression, and iron stores. In addition, a negative correlation was found between pparα expression, TBARS, carbonyl protein, and iron stores. In conclusion, our results suggest that the increase observed in the transport of lipids in the bloodstream and the decreased fatty acid oxidation in rats, which was promoted by iron dextran, might be attributed to increased oxidative stress.
url http://dx.doi.org/10.1155/2015/272617
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AT joyceferreiradacostaguerra irondextranincreaseshepaticoxidativestressandaltersexpressionofgenesrelatedtolipidmetabolismcontributingtohyperlipidaemiainmurinemodel
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