Positive selection of skeleton-related genes during duck domestication revealed by whole genome sequencing

• Main Authors: Tao Zhu, Xin Qi, Yu Chen, Liang Wang, Xueze Lv, Weifang Yang, Jianwei Zhang, Kaiyang Li, Zhonghua Ning, Zhihua Jiang, Lujiang Qu
• Format: Article
• Language: English
• Published: BMC 2021-09-01
• Series: BMC Ecology and Evolution
• Subjects: Duck; Domestication; Artificial selection; Positive selection; Skeleton
• Online Access: https://doi.org/10.1186/s12862-021-01894-7

Abstract Background Domestication alters several phenotypic, neurological, and physiological traits in domestic animals compared to those in their wild ancestors. Domestic ducks originated from mallards, and some studies have shown that spot-billed ducks may have also made minor genetic contributions to domestication. Compared with the two ancestral species, domestic ducks generally differ in body size and bone morphology. In this study, we performed both genomic and transcriptomic analyses to identify candidate genes for elucidating the genetic mechanisms underlying phenotypic variation. Methods In this study, the duck genome data from eight domestic breeds and two wild species were collected to study the genetic changes during domestication. And the transcriptome data of different tissues from wild ducks and seven domestic ducks were used to reveal the expression difference between wild and domestic ducks. Results Using fixation index (Fst) algorithm and transcriptome data, we found that the genes related to skeletal development had high Fst values in wild and domestic breeds, and the differentially expressed genes were mainly enriched in the ossification pathway. Our data strongly suggest that the skeletal systems of domestic ducks were changed to adapt to artificial selection for larger sizes. In addition, by combining the genome and transcriptome data, we found that some Fst candidate genes exhibited different expression patterns, and these genes were found to be involved in digestive, immune, and metabolic functions. Conclusions A wide range of phenotypic differences exists between domestic and wild ducks. Through both genome and transcriptome analyses, we found that genes related to the skeletal system in domestic ducks were strongly selected. Our findings provide new insight into duck domestication and selection effects during the domestication.