Bioactivity of Samsum ant (<it>Pachycondyla sennaarensis</it>) venom against lipopolysaccharides through antioxidant and upregulation of Akt1 signaling in rats

• Main Authors: Ebaid Hossam, Al-Khalifa Mohamed, Isa Ahmed M, Gadoa Saad
• Format: Article
• Language: English
• Published: BMC 2012-07-01
• Series: Lipids in Health and Disease
• Subjects: AKT1; Samsum ant venom; Oxidative stress; Anti-inflammatory; Lipopolysaccharides
• Online Access: http://www.lipidworld.com/content/11/1/93

<p>Abstract</p> <p>Background</p> <p>This study aimed at investigating the oxidative stress ameliorating effect, lipids profile restoration, and the anti-inflammatory effect of Samsum Ant Venom (SAV) in induced endotoxemic male rats, injected with bacterial lipopolysaccharides (LPS).</p> <p>Results</p> <p>Results revealed that LPS significantly increased the oxidative stress indications in LPS-injected rats. A significant increase of both malondialdehyde (MDA), and advanced oxidative protein products (AOPP), as well as a significant suppression of glutathione were all detected. Treatment with 100 μg/kg dose of SAV significantly restored the oxidative stress normal indications and increased the total glutathione levels. Treatment of the LPS-rats with 100 μg/kg dose of SAV showed a clear anti-inflammatory function; as the histological architecture of the hepatic tissue was partially recovered, along with a valuable decrease in the leukocytes infiltrated the hepatic tissues. Treatment of some rat groups with 600 μg/kg dose of SAV after LPS injection induced a severe endotoxemia that resulted in very high mortality rates. SAV versus the effects of LPS on AKT1, Fas, TNF-α and IFN-γ mRNA expression. SAV was found to significantly lower Fas gene expression comparing to the LPS group and restore the level of IFN-γ mRNA expression to that of the control group.</p> <p>Conclusion</p> <p>In conclusion, SAV, at the dose of 100 μg/kg body weight, maintained and restored the oxidative stability, the anti-inflammatory, and the hypolipidemic bioactivity in rats after induced disruption of these parameters by LPS injection. This improvement by SAV was mediated by upregulation of AKT1.</p>