Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion w...
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2011-03-01
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doaj-6c517e7d229f48ca91c40af9d6c1fceb2020-11-25T00:50:40ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052011-03-0142130330910.1590/S1517-83822011000100039Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patientsWafaa M El-EmshatyDouaa RaafatDoaa M ElghannamNiveen SaudyEhab E EltorabyAbd Elhameed A MetwalliNosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on maintenance haemodialysis. The results obtained in the present study revealed HCV infection of 56.4%. Combined Ag/Ab test detected 3 out of the 4 anti-HCV negative viraemic patients who were in the window period. The sensitivity, specificity and accuracy of the test were higher than that of anti HCV antibodies detection test (95.45%, 94.1% and 94.87% versus 81.8%, 88.23% and 84.6%) and they were raised to 100% on combining its positivity with liver enzymes elevation results. Therefore, this simple combined Ag/Ab test can be applied for early detection of HCV infection during window period among HD patients as an alternative to HCV RNA detection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039EgypthaemodialysisHCVHCV core antigenimmunoassay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wafaa M El-Emshaty Douaa Raafat Doaa M Elghannam Niveen Saudy Ehab E Eltoraby Abd Elhameed A Metwalli |
spellingShingle |
Wafaa M El-Emshaty Douaa Raafat Doaa M Elghannam Niveen Saudy Ehab E Eltoraby Abd Elhameed A Metwalli Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients Brazilian Journal of Microbiology Egypt haemodialysis HCV HCV core antigen immunoassay |
author_facet |
Wafaa M El-Emshaty Douaa Raafat Doaa M Elghannam Niveen Saudy Ehab E Eltoraby Abd Elhameed A Metwalli |
author_sort |
Wafaa M El-Emshaty |
title |
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients |
title_short |
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients |
title_full |
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients |
title_fullStr |
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients |
title_full_unstemmed |
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients |
title_sort |
diagnostic performance of an immunoassay for simultaneous detection of hcv core antigen and antibodies among haemodialysis patients |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2011-03-01 |
description |
Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on maintenance haemodialysis. The results obtained in the present study revealed HCV infection of 56.4%. Combined Ag/Ab test detected 3 out of the 4 anti-HCV negative viraemic patients who were in the window period. The sensitivity, specificity and accuracy of the test were higher than that of anti HCV antibodies detection test (95.45%, 94.1% and 94.87% versus 81.8%, 88.23% and 84.6%) and they were raised to 100% on combining its positivity with liver enzymes elevation results. Therefore, this simple combined Ag/Ab test can be applied for early detection of HCV infection during window period among HD patients as an alternative to HCV RNA detection. |
topic |
Egypt haemodialysis HCV HCV core antigen immunoassay |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039 |
work_keys_str_mv |
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