Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients

Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion w...

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Main Authors: Wafaa M El-Emshaty, Douaa Raafat, Doaa M Elghannam, Niveen Saudy, Ehab E Eltoraby, Abd Elhameed A Metwalli
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2011-03-01
Series:Brazilian Journal of Microbiology
Subjects:
HCV
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039
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spelling doaj-6c517e7d229f48ca91c40af9d6c1fceb2020-11-25T00:50:40ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052011-03-0142130330910.1590/S1517-83822011000100039Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patientsWafaa M El-EmshatyDouaa RaafatDoaa M ElghannamNiveen SaudyEhab E EltorabyAbd Elhameed A MetwalliNosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on maintenance haemodialysis. The results obtained in the present study revealed HCV infection of 56.4%. Combined Ag/Ab test detected 3 out of the 4 anti-HCV negative viraemic patients who were in the window period. The sensitivity, specificity and accuracy of the test were higher than that of anti HCV antibodies detection test (95.45%, 94.1% and 94.87% versus 81.8%, 88.23% and 84.6%) and they were raised to 100% on combining its positivity with liver enzymes elevation results. Therefore, this simple combined Ag/Ab test can be applied for early detection of HCV infection during window period among HD patients as an alternative to HCV RNA detection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039EgypthaemodialysisHCVHCV core antigenimmunoassay
collection DOAJ
language English
format Article
sources DOAJ
author Wafaa M El-Emshaty
Douaa Raafat
Doaa M Elghannam
Niveen Saudy
Ehab E Eltoraby
Abd Elhameed A Metwalli
spellingShingle Wafaa M El-Emshaty
Douaa Raafat
Doaa M Elghannam
Niveen Saudy
Ehab E Eltoraby
Abd Elhameed A Metwalli
Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
Brazilian Journal of Microbiology
Egypt
haemodialysis
HCV
HCV core antigen
immunoassay
author_facet Wafaa M El-Emshaty
Douaa Raafat
Doaa M Elghannam
Niveen Saudy
Ehab E Eltoraby
Abd Elhameed A Metwalli
author_sort Wafaa M El-Emshaty
title Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
title_short Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
title_full Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
title_fullStr Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
title_full_unstemmed Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
title_sort diagnostic performance of an immunoassay for simultaneous detection of hcv core antigen and antibodies among haemodialysis patients
publisher Sociedade Brasileira de Microbiologia
series Brazilian Journal of Microbiology
issn 1517-8382
1678-4405
publishDate 2011-03-01
description Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on maintenance haemodialysis. The results obtained in the present study revealed HCV infection of 56.4%. Combined Ag/Ab test detected 3 out of the 4 anti-HCV negative viraemic patients who were in the window period. The sensitivity, specificity and accuracy of the test were higher than that of anti HCV antibodies detection test (95.45%, 94.1% and 94.87% versus 81.8%, 88.23% and 84.6%) and they were raised to 100% on combining its positivity with liver enzymes elevation results. Therefore, this simple combined Ag/Ab test can be applied for early detection of HCV infection during window period among HD patients as an alternative to HCV RNA detection.
topic Egypt
haemodialysis
HCV
HCV core antigen
immunoassay
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039
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