Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study

Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to det...

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Main Authors: Emine Akçay, Ahmet Çarhan, Gözde Hondur, Zeliha Koçak Tufan, Necati Duru, Selçuk Kılıç, Ezgi Naz Ensari, Nagihan Uğurlu, Nurullah Çağıl
Format: Article
Language:English
Published: Elsevier 2017-07-01
Series:Brazilian Journal of Infectious Diseases
Online Access:http://www.sciencedirect.com/science/article/pii/S1413867016302069
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spelling doaj-6c4594c80d4a4ef094ca628516188c6e2020-11-25T03:25:17ZengElsevierBrazilian Journal of Infectious Diseases1413-86702017-07-01214391395S1413-86702017000400391Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled studyEmine Akçay0Ahmet Çarhan1Gözde Hondur2Zeliha Koçak Tufan3Necati Duru4Selçuk Kılıç5Ezgi Naz Ensari6Nagihan Uğurlu7Nurullah Çağıl8Yıldırım Beyazıt, University Faculty of Medicine, Department of Ophthalmology, Ankara, TurkeyYıldırım Beyazıt, University Faculty of Medicine, Department of Medical Biology, Ankara, TurkeyUlucanlar Eye Training and Research Hospital, Ankara, TurkeyYıldırım Beyazıt University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Ankara, TurkeyKayseri Education and Research Hospital, Ophthalmology Clinic, Kayseri, TurkeyTurkey Public Health Institute, Ankara, TurkeyYıldırım Beyazıt, University Faculty of Medicine, Department of Ophthalmology, Ankara, Turkey; Corresponding author.Yıldırım Beyazıt, University Faculty of Medicine, Department of Ophthalmology, Ankara, TurkeyYıldırım Beyazıt, University Faculty of Medicine, Department of Ophthalmology, Ankara, TurkeyBackground: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at −80 ̊C up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used for control group. Results: A total of 176 samples (conjunctival and pharyngeal samples of 62 patient and 26 healthy subjects) were included. The mean age of 34 (55.7%) male and 27 (44.3%) female patients was 34 ± 17. Twenty five (40.3%) of the patients were receiving antibiotic drops at first visit. The main etiologic agent in conjunctival samples was found to be Adenovirus (46/62, 74.2%) followed by Enterovirus 70 (4/62, 6.4%) and Enterovirus 71 (4/62, 6.4%). Coxsackievirus 16 and 24 were also found in 2 patients (1/62 each, 1.6%). Pharyngeal samples were also positive for Adenovirus (20/62, 32.3%), Enterovirus 70 and 71 (7/62, 11.3% and 5/62, 8.1% respectively), Coxsackievirus 16 and 24 (2/62, 3.2% and 1/61, 1.6%). Conclusions: It is very difficult in viral conjunctivitis to make clinical differentiation caused by different agents because of common clinical signs and symptoms. In routine clinical work, the viral conjunctivitis usually related with Adenovirus. But almost one fourth of the patients’ conjunctivitis were not related to Adenovirus, which shows the importance of the laboratory diagnostics. True diagnosis plays an important role on prevention of contamination and unnecessary use of antibiotics in viral conjunctivitis. Keywords: Adenovirus, Conjunctivitis, Viral, PCRhttp://www.sciencedirect.com/science/article/pii/S1413867016302069
collection DOAJ
language English
format Article
sources DOAJ
author Emine Akçay
Ahmet Çarhan
Gözde Hondur
Zeliha Koçak Tufan
Necati Duru
Selçuk Kılıç
Ezgi Naz Ensari
Nagihan Uğurlu
Nurullah Çağıl
spellingShingle Emine Akçay
Ahmet Çarhan
Gözde Hondur
Zeliha Koçak Tufan
Necati Duru
Selçuk Kılıç
Ezgi Naz Ensari
Nagihan Uğurlu
Nurullah Çağıl
Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
Brazilian Journal of Infectious Diseases
author_facet Emine Akçay
Ahmet Çarhan
Gözde Hondur
Zeliha Koçak Tufan
Necati Duru
Selçuk Kılıç
Ezgi Naz Ensari
Nagihan Uğurlu
Nurullah Çağıl
author_sort Emine Akçay
title Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
title_short Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
title_full Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
title_fullStr Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
title_full_unstemmed Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
title_sort molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
publisher Elsevier
series Brazilian Journal of Infectious Diseases
issn 1413-8670
publishDate 2017-07-01
description Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at −80 ̊C up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used for control group. Results: A total of 176 samples (conjunctival and pharyngeal samples of 62 patient and 26 healthy subjects) were included. The mean age of 34 (55.7%) male and 27 (44.3%) female patients was 34 ± 17. Twenty five (40.3%) of the patients were receiving antibiotic drops at first visit. The main etiologic agent in conjunctival samples was found to be Adenovirus (46/62, 74.2%) followed by Enterovirus 70 (4/62, 6.4%) and Enterovirus 71 (4/62, 6.4%). Coxsackievirus 16 and 24 were also found in 2 patients (1/62 each, 1.6%). Pharyngeal samples were also positive for Adenovirus (20/62, 32.3%), Enterovirus 70 and 71 (7/62, 11.3% and 5/62, 8.1% respectively), Coxsackievirus 16 and 24 (2/62, 3.2% and 1/61, 1.6%). Conclusions: It is very difficult in viral conjunctivitis to make clinical differentiation caused by different agents because of common clinical signs and symptoms. In routine clinical work, the viral conjunctivitis usually related with Adenovirus. But almost one fourth of the patients’ conjunctivitis were not related to Adenovirus, which shows the importance of the laboratory diagnostics. True diagnosis plays an important role on prevention of contamination and unnecessary use of antibiotics in viral conjunctivitis. Keywords: Adenovirus, Conjunctivitis, Viral, PCR
url http://www.sciencedirect.com/science/article/pii/S1413867016302069
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