Microarrays, deep sequencing and the true measure of the transcriptome

<p>Abstract</p> <p>Microarrays first made the analysis of the transcriptome possible, and have produced much important information. Today, however, researchers are increasingly turning to direct high-throughput sequencing - RNA-Seq - which has considerable advantages for examining...

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Main Authors: Oliver Brian, Malone John H
Format: Article
Language:English
Published: BMC 2011-05-01
Series:BMC Biology
Online Access:http://www.biomedcentral.com/1741-7007/9/34
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spelling doaj-6c34bac134b84ef6b464fa03ad60a83d2020-11-24T21:19:08ZengBMCBMC Biology1741-70072011-05-01913410.1186/1741-7007-9-34Microarrays, deep sequencing and the true measure of the transcriptomeOliver BrianMalone John H<p>Abstract</p> <p>Microarrays first made the analysis of the transcriptome possible, and have produced much important information. Today, however, researchers are increasingly turning to direct high-throughput sequencing - RNA-Seq - which has considerable advantages for examining transcriptome fine structure - for example in the detection of allele-specific expression and splice junctions. In this article, we discuss the relative merits of the two techniques, the inherent biases in each, and whether all of the vast body of array work needs to be revisited using the newer technology. We conclude that microarrays remain useful and accurate tools for measuring expression levels, and RNA-Seq complements and extends microarray measurements.</p> http://www.biomedcentral.com/1741-7007/9/34
collection DOAJ
language English
format Article
sources DOAJ
author Oliver Brian
Malone John H
spellingShingle Oliver Brian
Malone John H
Microarrays, deep sequencing and the true measure of the transcriptome
BMC Biology
author_facet Oliver Brian
Malone John H
author_sort Oliver Brian
title Microarrays, deep sequencing and the true measure of the transcriptome
title_short Microarrays, deep sequencing and the true measure of the transcriptome
title_full Microarrays, deep sequencing and the true measure of the transcriptome
title_fullStr Microarrays, deep sequencing and the true measure of the transcriptome
title_full_unstemmed Microarrays, deep sequencing and the true measure of the transcriptome
title_sort microarrays, deep sequencing and the true measure of the transcriptome
publisher BMC
series BMC Biology
issn 1741-7007
publishDate 2011-05-01
description <p>Abstract</p> <p>Microarrays first made the analysis of the transcriptome possible, and have produced much important information. Today, however, researchers are increasingly turning to direct high-throughput sequencing - RNA-Seq - which has considerable advantages for examining transcriptome fine structure - for example in the detection of allele-specific expression and splice junctions. In this article, we discuss the relative merits of the two techniques, the inherent biases in each, and whether all of the vast body of array work needs to be revisited using the newer technology. We conclude that microarrays remain useful and accurate tools for measuring expression levels, and RNA-Seq complements and extends microarray measurements.</p>
url http://www.biomedcentral.com/1741-7007/9/34
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