One-Pot Green Synthesis and Bioapplication of<smcaps>l</smcaps>-Arginine-Capped Superparamagnetic Fe₃O₄ Nanoparticles

• Main Authors: Lai Yongchao, Yin Weiwei, Liu Jinting, Xi Rimo, Zhan Jinhua
• Format: Article
• Language: English
• Published: SpringerOpen 2009-01-01
• Series: Nanoscale Research Letters
• Subjects: Magnetite; Superparamagnetic; Solvothermal; Amino acid; Nanocrystals
• Online Access: http://dx.doi.org/10.1007/s11671-009-9480-x

<p>Abstract</p> <p>Water-soluble<smcaps>l</smcaps>-arginine-capped Fe₃O₄ nanoparticles were synthesized using a one-pot and green method. Nontoxic, renewable and inexpensive reagents including FeCl₃,<smcaps>l</smcaps>-arginine, glycerol and water were chosen as raw materials. Fe₃O₄ nanoparticles show different dispersive states in acidic and alkaline solutions for the two distinct forms of surface binding<smcaps>l</smcaps>-arginine. Powder X-ray diffraction and X-ray photoelectron spectroscopy were used to identify the structure of Fe₃O₄ nanocrystals. The products behave like superparamagnetism at room temperature with saturation magnetization of 49.9 emu g^&#8722;1 and negligible remanence or coercivity. In the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, the anti-chloramphenicol monoclonal antibodies were connected to the<smcaps>l</smcaps>-arginine-capped magnetite nanoparticles. The as-prepared conjugates could be used in immunomagnetic assay.</p> <p>(See supplementary material <supplr sid="S1">1</supplr>)</p> <suppl id="S1"> <title> <p>Electronic supplementary material</p> </title> <text> <p>The online version of this article (doi:10.1007/s11671-009-9480-x) contains supplementary material, which is available to authorized users.</p> </text> <file name="1556-276X-5-302-S1.pdf"> <p>Click here for file</p> </file> </suppl>