A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity
With the development of magnetic manipulation technology based on magnetic nanoparticles (MNPs), scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells. The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2021-11-01
|
| Series: | Bioactive Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21001080 |
| id |
doaj-6bfdf2fe20ff4267b98238aaed82f1dc |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yuezhi Lu Chun-Hua Yu Guangzheng Yang Ningjia Sun Fei Jiang Mingliang Zhou Xiaolin Wu Jiaxin Luo Cui Huang Wenjie Zhang Xinquan Jiang |
| spellingShingle |
Yuezhi Lu Chun-Hua Yu Guangzheng Yang Ningjia Sun Fei Jiang Mingliang Zhou Xiaolin Wu Jiaxin Luo Cui Huang Wenjie Zhang Xinquan Jiang A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity Bioactive Materials Microtissue Magnetic nanoparticle Stem cell Human umbilical vein endothelial cell Vascularization |
| author_facet |
Yuezhi Lu Chun-Hua Yu Guangzheng Yang Ningjia Sun Fei Jiang Mingliang Zhou Xiaolin Wu Jiaxin Luo Cui Huang Wenjie Zhang Xinquan Jiang |
| author_sort |
Yuezhi Lu |
| title |
A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| title_short |
A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| title_full |
A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| title_fullStr |
A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| title_full_unstemmed |
A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| title_sort |
rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacity |
| publisher |
KeAi Communications Co., Ltd. |
| series |
Bioactive Materials |
| issn |
2452-199X |
| publishDate |
2021-11-01 |
| description |
With the development of magnetic manipulation technology based on magnetic nanoparticles (MNPs), scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells. The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues. Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs. Herein, safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h, and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled, layer by layer, in 5 min intervals. The in vivo transplantation results showed that in a stem cell microtissue with trisection architecture, the two separated human umbilical vein endothelial cell (HUVEC) layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels, which anastomosed with the host vasculature. The “hamburger” architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival. This study will contribute to the construction and application of structural and functional tissues or organs in the future. |
| topic |
Microtissue Magnetic nanoparticle Stem cell Human umbilical vein endothelial cell Vascularization |
| url |
http://www.sciencedirect.com/science/article/pii/S2452199X21001080 |
| work_keys_str_mv |
AT yuezhilu arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT chunhuayu arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT guangzhengyang arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT ningjiasun arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT feijiang arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT mingliangzhou arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT xiaolinwu arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT jiaxinluo arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT cuihuang arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT wenjiezhang arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT xinquanjiang arapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT yuezhilu rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT chunhuayu rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT guangzhengyang rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT ningjiasun rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT feijiang rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT mingliangzhou rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT xiaolinwu rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT jiaxinluo rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT cuihuang rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT wenjiezhang rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity AT xinquanjiang rapidlymagneticallyassembledstemcellmicrotissuewithhamburgerarchitectureandenhancedvascularizationcapacity |
| _version_ |
1721209874953535488 |
| spelling |
doaj-6bfdf2fe20ff4267b98238aaed82f1dc2021-08-12T04:35:04ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-11-0161137563765A rapidly magnetically assembled stem cell microtissue with “hamburger” architecture and enhanced vascularization capacityYuezhi Lu0Chun-Hua Yu1Guangzheng Yang2Ningjia Sun3Fei Jiang4Mingliang Zhou5Xiaolin Wu6Jiaxin Luo7Cui Huang8Wenjie Zhang9Xinquan Jiang10Department of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, 430079, ChinaDepartment of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Corresponding author.Department of Prosthodontics, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Corresponding author.With the development of magnetic manipulation technology based on magnetic nanoparticles (MNPs), scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells. The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues. Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs. Herein, safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h, and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled, layer by layer, in 5 min intervals. The in vivo transplantation results showed that in a stem cell microtissue with trisection architecture, the two separated human umbilical vein endothelial cell (HUVEC) layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels, which anastomosed with the host vasculature. The “hamburger” architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival. This study will contribute to the construction and application of structural and functional tissues or organs in the future.http://www.sciencedirect.com/science/article/pii/S2452199X21001080MicrotissueMagnetic nanoparticleStem cellHuman umbilical vein endothelial cellVascularization |