Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat...

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Bibliographic Details
Main Authors: Mark Dizon, Marek Tatarko, Tibor Hianik
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Sensors
Subjects:
plasmin
trypsin
protease
casein
cleavage
acoustic sensor
Online Access:https://www.mdpi.com/1424-8220/20/19/5594
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spelling doaj-6bdac84ba47a4df8a3d516769dd30aa62020-11-25T01:46:22ZengMDPI AGSensors1424-82202020-09-01205594559410.3390/s20195594Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic MethodsMark Dizon0Marek Tatarko1Tibor Hianik2School of Chemistry, University College Dublin, Belfield, Dublin 4, IrelandDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, SlovakiaThis review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.https://www.mdpi.com/1424-8220/20/19/5594plasmintrypsinproteasecaseincleavageacoustic sensor
collection DOAJ
language English
format Article
sources DOAJ
author Mark Dizon
Marek Tatarko
Tibor Hianik
spellingShingle Mark Dizon
Marek Tatarko
Tibor Hianik
Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
Sensors
plasmin
trypsin
protease
casein
cleavage
acoustic sensor
author_facet Mark Dizon
Marek Tatarko
Tibor Hianik
author_sort Mark Dizon
title Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
title_short Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
title_full Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
title_fullStr Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
title_full_unstemmed Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
title_sort advances in analysis of milk proteases activity at surfaces and in a volume by acoustic methods
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2020-09-01
description This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.
topic plasmin
trypsin
protease
casein
cleavage
acoustic sensor
url https://www.mdpi.com/1424-8220/20/19/5594
work_keys_str_mv AT markdizon advancesinanalysisofmilkproteasesactivityatsurfacesandinavolumebyacousticmethods
AT marektatarko advancesinanalysisofmilkproteasesactivityatsurfacesandinavolumebyacousticmethods
AT tiborhianik advancesinanalysisofmilkproteasesactivityatsurfacesandinavolumebyacousticmethods
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