Determination of pyrazinamide in human by high performance liquid chromatography.

Determination of pyrazinamide in human by high performance liquid chromatography.

A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1...

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Bibliographic Details
Main Authors: Revankar S, Desai N, Vaidya A, Bhatt A, Anjaneyulu B
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 1994-01-01
Series:Journal of Postgraduate Medicine
Subjects:
Adult
Antitubercular Agents
blood
Biological Availability
Chromatography
High Pressure Liquid
methods
standards
Human
Male
Niacinamide
Pyrazinamide
pharmacokinetics
Reproducibility of Results
Online Access:http://www.jpgmonline.com/article.asp?issn=0022-3859;year=1994;volume=40;issue=1;spage=7;epage=9;aulast=Revankar
Description
Summary:A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1M NaOH and injected onto HPLC. The separation of pyrazinamide and the internal standard is carried out on a Supelco LC-18 (DB) column with a basic mobile phase. Pyrazinoic acid, the major metabolite, other anti-tuberculous drugs and endogenous components do not interfere with measurement of pyrazinamide. The limit of detection of pyrazinamide with this method is 0.2 mg/0.2 ml plasma (CV 8.2%).
ISSN:0022-3859
0972-2823

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