INCREASED URINARY NEOPTERIN: CREATININE RATIO AS A MARKER OF ACTIVATION OF CELL-MEDIATED IMMUNITY AND OXIDATIVE STRESS IN THE IRANIAN PATIENTS WITH MULTIPLE SCLEROSIS

• Main Authors: H. Khorami, TR. Neyestani, Mehri. Kadkhodaee, J. Lotfi
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2003-09-01
• Series: Iranian Journal of Allergy, Asthma and Immunology
• Subjects: Neopterin; Cell-Mediated Immunity
• Online Access: https://ijaai.tums.ac.ir/index.php/ijaai/article/view/49

Neopterin, apyrazinopyrimidine compound, is produced by macrophages after induction by interferon gamma (IFN-y) and serves as a marker of cellular immune system activation followed by oxidative stress. The aim of this study was to determine urinary neopterin to creatinine ratio (UNCR) as a surrogate marker of cell-mediated immune activation in multiple sclerosis (MS). Three weekly early morning urine samples were collected from 27 patients with MS and 31 age- and sex-matched apparently healthy subjects. Urinary neopterin and creatinine were determined using reversed phase high-performance liquid chromatography and Jaffe reaction, respectively. UNCR was significantly higher in patients than in healthy controls indicating IFN-y-induced cellular immunity activation and oxidative stress in multiple sclerosis. As a non-invasive method, UNCR determination may be helpful in monitoring disease progression and the effects of therapies, as well.