Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase

The downscale of different unit operations for the biocatalytic carboligation of benzaldehyde and acetaldehyde catalyzed by benzoylformate decarboxylase from Pseudomonas putida was investigated. The reactor volume was reduced to 115 μl thus enabling a substrate and enzyme saving by a factor of 52 in...

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Main Authors:	Fagaschewski Janosch, Bohne Sven, Kaufhold Dennis, Müller Jörg, Hilterhaus Lutz
Format:	Article
Language:	English
Published:	De Gruyter 2012-08-01
Series:	Green Processing and Synthesis
Subjects:	biocatalysis downscale enzyme membrane screening
Online Access:	https://doi.org/10.1515/gps-2012-0013

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spelling	doaj-6b9f11a6ebdb4229a02807f37a3642b42021-10-02T17:47:22ZengDe GruyterGreen Processing and Synthesis2191-95422191-95502012-08-011433734410.1515/gps-2012-0013Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylaseFagaschewski Janosch0Bohne Sven1Kaufhold Dennis2Müller Jörg3Hilterhaus Lutz4Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, D-21073 Hamburg, GermanyInstitute of Microsystems Technology, Hamburg University of Technology, Eissendorfer Strasse 42, D-21073 Hamburg, GermanyInstitute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, D-21073 Hamburg, GermanyInstitute of Microsystems Technology, Hamburg University of Technology, Eissendorfer Strasse 42, D-21073 Hamburg, GermanyInstitute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, D-21073 Hamburg, GermanyThe downscale of different unit operations for the biocatalytic carboligation of benzaldehyde and acetaldehyde catalyzed by benzoylformate decarboxylase from Pseudomonas putida was investigated. The reactor volume was reduced to 115 μl thus enabling a substrate and enzyme saving by a factor of 52 in comparison to standard laboratory techniques. Additionally, the successful downscale of membrane based liquid-liquid contactors was shown, which allows, for example, the screening of solvents for extraction as well as the feed of a substrate. Here, comparable volumes as well as residence times were realized, enabling the integration of all three unit operations.https://doi.org/10.1515/gps-2012-0013biocatalysisdownscaleenzymemembranescreening
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Fagaschewski Janosch Bohne Sven Kaufhold Dennis Müller Jörg Hilterhaus Lutz
spellingShingle	Fagaschewski Janosch Bohne Sven Kaufhold Dennis Müller Jörg Hilterhaus Lutz Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase Green Processing and Synthesis biocatalysis downscale enzyme membrane screening
author_facet	Fagaschewski Janosch Bohne Sven Kaufhold Dennis Müller Jörg Hilterhaus Lutz
author_sort	Fagaschewski Janosch
title	Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
title_short	Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
title_full	Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
title_fullStr	Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
title_full_unstemmed	Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
title_sort	modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
publisher	De Gruyter
series	Green Processing and Synthesis
issn	2191-9542 2191-9550
publishDate	2012-08-01
description	The downscale of different unit operations for the biocatalytic carboligation of benzaldehyde and acetaldehyde catalyzed by benzoylformate decarboxylase from Pseudomonas putida was investigated. The reactor volume was reduced to 115 μl thus enabling a substrate and enzyme saving by a factor of 52 in comparison to standard laboratory techniques. Additionally, the successful downscale of membrane based liquid-liquid contactors was shown, which allows, for example, the screening of solvents for extraction as well as the feed of a substrate. Here, comparable volumes as well as residence times were realized, enabling the integration of all three unit operations.
topic	biocatalysis downscale enzyme membrane screening
url	https://doi.org/10.1515/gps-2012-0013
work_keys_str_mv	AT fagaschewskijanosch modularmicroreactionengineeringforcarboligationcatalyzedbybenzoylformatedecarboxylase AT bohnesven modularmicroreactionengineeringforcarboligationcatalyzedbybenzoylformatedecarboxylase AT kaufholddennis modularmicroreactionengineeringforcarboligationcatalyzedbybenzoylformatedecarboxylase AT mullerjorg modularmicroreactionengineeringforcarboligationcatalyzedbybenzoylformatedecarboxylase AT hilterhauslutz modularmicroreactionengineeringforcarboligationcatalyzedbybenzoylformatedecarboxylase
_version_	1716850473199206400

Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase

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