A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran
Background: Type 2 diabetes mellitus is a multifactorial and heterogenic disease with a complex etiology. In recent decades the association of a large number of genes has been shown with T2DM. CAPN10 gene was the first T2DM candidate gene identified through genome-wide screening and positional cloni...
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doaj-6b78dfedbe8c4e3bbc61225d440865dc2020-11-25T00:03:07ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302018-10-01194403407A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of IranAbdollah Gravand0Ali Mohammad Foroughmand1Mehdi Pourmehdi Boroujeni2Department of Genetics, Faculty of Science, Shahid Chamran University, Ahvaz, IranDepartment of Genetics, Faculty of Science, Shahid Chamran University, Ahvaz, Iran; Corresponding author.Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IranBackground: Type 2 diabetes mellitus is a multifactorial and heterogenic disease with a complex etiology. In recent decades the association of a large number of genes has been shown with T2DM. CAPN10 gene was the first T2DM candidate gene identified through genome-wide screening and positional cloning, and among all identified genes until now, TCF7L2 gene has shown most association with T2DM. The aim of this study was to investigate the association between TCF7L2 rs11196205(C/G) and CAPN10 rs3792267 (G/A) with T2DM in a subset of Iranian population from Khuzestan province. It should be noted that this is the first report of TCF7L2 polymorphism rs11196205with T2DM in Iran. Subjects and methods: A case-control association study was performed using 150 T2DM patients and 150 controls. Genotyping for TCF7L2 rs11196205 was done by Tetra-Primer ARMS-PCR and for CAPN10 rs3792267 was done by PCR-RFLP Technique. Results: Statistical analyses were carried out using SPSS version 16. In examining TCF7L2 rs11196205 based on the genotype GG, results for CG genotype were, 95%CI = (0.5–1.7), OR = 0.92, P-value = 0.79 and for genotype CC were, 95%CI = (0.94–3.92), OR = 1.92, P-value = 0.07. in examining CAPN10 rs3792267 based on the genotype AA, results for GG genotype were, 95%CI = (0.55–6.8), OR = 1.93, P-value = 0.31 and for genotype GA were, 95%CI = (0.43–5.64), OR = 1.55, P-value = 0.5. So, in both polymorphisms, none of the alleles or genotypes had significant statistical differences between case and control groups (P > 0.05). Conclusion: Our results showed that TCF7L2 rs11196205 and CAPN10 rs3792267 (SNP- 43) polymorphisms are not associated with the risk of T2DM in the studied population. Keywords: Association study, CAPN10, TCF7L2, Type 2 diabetes mellitus, rs11196205, rs3792267http://www.sciencedirect.com/science/article/pii/S111086301830020X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abdollah Gravand Ali Mohammad Foroughmand Mehdi Pourmehdi Boroujeni |
spellingShingle |
Abdollah Gravand Ali Mohammad Foroughmand Mehdi Pourmehdi Boroujeni A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran Egyptian Journal of Medical Human Genetics |
author_facet |
Abdollah Gravand Ali Mohammad Foroughmand Mehdi Pourmehdi Boroujeni |
author_sort |
Abdollah Gravand |
title |
A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran |
title_short |
A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran |
title_full |
A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran |
title_fullStr |
A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran |
title_full_unstemmed |
A study on the association of TCF7L2 rs11196205 (C/G) and CAPN10 rs3792267 (G/A) polymorphisms with type 2 diabetes mellitus in the South Western of Iran |
title_sort |
study on the association of tcf7l2 rs11196205 (c/g) and capn10 rs3792267 (g/a) polymorphisms with type 2 diabetes mellitus in the south western of iran |
publisher |
SpringerOpen |
series |
Egyptian Journal of Medical Human Genetics |
issn |
1110-8630 |
publishDate |
2018-10-01 |
description |
Background: Type 2 diabetes mellitus is a multifactorial and heterogenic disease with a complex etiology. In recent decades the association of a large number of genes has been shown with T2DM. CAPN10 gene was the first T2DM candidate gene identified through genome-wide screening and positional cloning, and among all identified genes until now, TCF7L2 gene has shown most association with T2DM. The aim of this study was to investigate the association between TCF7L2 rs11196205(C/G) and CAPN10 rs3792267 (G/A) with T2DM in a subset of Iranian population from Khuzestan province. It should be noted that this is the first report of TCF7L2 polymorphism rs11196205with T2DM in Iran. Subjects and methods: A case-control association study was performed using 150 T2DM patients and 150 controls. Genotyping for TCF7L2 rs11196205 was done by Tetra-Primer ARMS-PCR and for CAPN10 rs3792267 was done by PCR-RFLP Technique. Results: Statistical analyses were carried out using SPSS version 16. In examining TCF7L2 rs11196205 based on the genotype GG, results for CG genotype were, 95%CI = (0.5–1.7), OR = 0.92, P-value = 0.79 and for genotype CC were, 95%CI = (0.94–3.92), OR = 1.92, P-value = 0.07. in examining CAPN10 rs3792267 based on the genotype AA, results for GG genotype were, 95%CI = (0.55–6.8), OR = 1.93, P-value = 0.31 and for genotype GA were, 95%CI = (0.43–5.64), OR = 1.55, P-value = 0.5. So, in both polymorphisms, none of the alleles or genotypes had significant statistical differences between case and control groups (P > 0.05). Conclusion: Our results showed that TCF7L2 rs11196205 and CAPN10 rs3792267 (SNP- 43) polymorphisms are not associated with the risk of T2DM in the studied population. Keywords: Association study, CAPN10, TCF7L2, Type 2 diabetes mellitus, rs11196205, rs3792267 |
url |
http://www.sciencedirect.com/science/article/pii/S111086301830020X |
work_keys_str_mv |
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