Quartz Crystal Microbalance Genosensing of <i>Brettanomyces bruxellensis</i> Yeast in Wine Using a Rapid and Efficient <i>Drop and Collect</i> Protocol

• Main Authors: Simone Poggesi, Lan Zhou, Giuliocesare Casari Bariani, Rakesh Mittapalli, Marisa Manzano, Rodica Elena Ionescu
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Crystals
• Subjects: quartz crystal microbalance; multi-TEMs patterned crystal; genosensing; <i>Brettanomyces bruxellensis</i>; <i>drop and collect</i> protocol
• Online Access: https://www.mdpi.com/2073-4352/11/5/562

A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from <i>Brettanomyces bruxellensis</i> (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient <i>drop and collect</i> protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/μL DNA of <i>B. bruxellensis</i> extracted from a wine sample (VR2008) is reported.