Hamp Type-1 Promotes Antimicrobial Defense via Direct Microbial Killing and Regulating Iron Metabolism in Grass Carp (<i>Ctenopharyngodon idella</i>)

• Main Authors: Yazhen Hu, Tomofumi Kurobe, Xiaoling Liu, Yong-An Zhang, Jianguo Su, Gailing Yuan
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: Biomolecules
• Subjects: Antibacterial; <i>Ctenopharyngodon idella</i>; hepcidin; host defense; iron homeostasis
• Online Access: https://www.mdpi.com/2218-273X/10/6/825

Hepcidin is an antimicrobial peptide and regulator of iron homeostasis which has two isoforms in most fishes and some mammals. Previous studies have reported that the two hepcidin isoforms have different roles. Hamp type-1 plays a regulatory role in iron metabolism and hamp type-2 mostly performs an antimicrobial role. In this study, we found that <i>Ctenopharyngodon idella</i> (<i>C. idella</i>) have only one hepcidin isoform (hamp type-1), which showed both broad-spectrum antibacterial and iron regulatory functions. <i>C. idella </i>hepcidin mature peptide (hepcidin-25) and truncated peptide (hepcidin-20) exhibited bactericidal activities against both Gram-positive and Gram-negative bacteria in a dose-dependent manner in part through membrane rupture and binding to bacterial genomic DNA. The data from challenge tests demonstrated that the administration of hepcidin-25 significantly reduced mortality rates of <i>C. idella</i> by <i>A. hydrophila</i> infection, probably due to direct bactericidal activities of the peptide and a reduction of iron content in the fish serum. In addition, a comparison between hepcidin-20 and -25 suggests that the N terminal 5 amino acids play a critical role in reducing iron content in fish serum. Our findings revealed an important role of hamp type-1 in maintaining iron homeostasis and fighting against bacterial infections, suggesting the hepcidin has implications for the prevention and control of bacterial infection in aquaculture.