Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection

Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desatur...

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Main Authors: Xue GAO, Xin-hua DU, Lu-pei ZHANG, Yan CHEN, Hui-jiang GAO, Shang-zhong XU, Jun-ya LI
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Journal of Integrative Agriculture
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311915612187
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spelling doaj-6b404d8a20f64f02a4cb800e7a22b60f2021-06-07T06:51:30ZengElsevierJournal of Integrative Agriculture2095-31192016-06-0115613301337Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injectionXue GAO0Xin-hua DU1Lu-pei ZHANG2Yan CHEN3Hui-jiang GAO4Shang-zhong XU5Jun-ya LI6Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Correspondence XU Shang-zhongKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaOmega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desaturase gene (fat-1 gene). Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias of Bfat-1 gene was increased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the ω-6/ω-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver (P<0.05), and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection (P<0.05). This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of ω-6 PUFAs to ω-3 PUFAs.http://www.sciencedirect.com/science/article/pii/S2095311915612187beef cattlecodon biasmodified Bfat-1hydrodynamic tail vein injectionmice
collection DOAJ
language English
format Article
sources DOAJ
author Xue GAO
Xin-hua DU
Lu-pei ZHANG
Yan CHEN
Hui-jiang GAO
Shang-zhong XU
Jun-ya LI
spellingShingle Xue GAO
Xin-hua DU
Lu-pei ZHANG
Yan CHEN
Hui-jiang GAO
Shang-zhong XU
Jun-ya LI
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
Journal of Integrative Agriculture
beef cattle
codon bias
modified Bfat-1
hydrodynamic tail vein injection
mice
author_facet Xue GAO
Xin-hua DU
Lu-pei ZHANG
Yan CHEN
Hui-jiang GAO
Shang-zhong XU
Jun-ya LI
author_sort Xue GAO
title Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
title_short Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
title_full Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
title_fullStr Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
title_full_unstemmed Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
title_sort modified bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
publisher Elsevier
series Journal of Integrative Agriculture
issn 2095-3119
publishDate 2016-06-01
description Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desaturase gene (fat-1 gene). Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias of Bfat-1 gene was increased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the ω-6/ω-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver (P<0.05), and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection (P<0.05). This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of ω-6 PUFAs to ω-3 PUFAs.
topic beef cattle
codon bias
modified Bfat-1
hydrodynamic tail vein injection
mice
url http://www.sciencedirect.com/science/article/pii/S2095311915612187
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