Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection
Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desatur...
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doaj-6b404d8a20f64f02a4cb800e7a22b60f2021-06-07T06:51:30ZengElsevierJournal of Integrative Agriculture2095-31192016-06-0115613301337Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injectionXue GAO0Xin-hua DU1Lu-pei ZHANG2Yan CHEN3Hui-jiang GAO4Shang-zhong XU5Jun-ya LI6Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Correspondence XU Shang-zhongKey Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaOmega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desaturase gene (fat-1 gene). Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias of Bfat-1 gene was increased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the ω-6/ω-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver (P<0.05), and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection (P<0.05). This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of ω-6 PUFAs to ω-3 PUFAs.http://www.sciencedirect.com/science/article/pii/S2095311915612187beef cattlecodon biasmodified Bfat-1hydrodynamic tail vein injectionmice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xue GAO Xin-hua DU Lu-pei ZHANG Yan CHEN Hui-jiang GAO Shang-zhong XU Jun-ya LI |
spellingShingle |
Xue GAO Xin-hua DU Lu-pei ZHANG Yan CHEN Hui-jiang GAO Shang-zhong XU Jun-ya LI Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection Journal of Integrative Agriculture beef cattle codon bias modified Bfat-1 hydrodynamic tail vein injection mice |
author_facet |
Xue GAO Xin-hua DU Lu-pei ZHANG Yan CHEN Hui-jiang GAO Shang-zhong XU Jun-ya LI |
author_sort |
Xue GAO |
title |
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
title_short |
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
title_full |
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
title_fullStr |
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
title_full_unstemmed |
Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
title_sort |
modified bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2016-06-01 |
description |
Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desaturase gene (fat-1 gene). Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias of Bfat-1 gene was increased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the ω-6/ω-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver (P<0.05), and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection (P<0.05). This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of ω-6 PUFAs to ω-3 PUFAs. |
topic |
beef cattle codon bias modified Bfat-1 hydrodynamic tail vein injection mice |
url |
http://www.sciencedirect.com/science/article/pii/S2095311915612187 |
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