A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

<p>Abstract</p> <p>Background</p> <p>Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an inc...

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Bibliographic Details
Main Authors: Orlova Nadezhda A, Orlov Alexandre V, Vorobiev Ivan I
Format: Article
Language:English
Published: BMC 2012-06-01
Series:BMC Research Notes
Subjects:
Molecular cloning
Software tool
Cloning technique
Hybrid restriction site
Online Access:http://www.biomedcentral.com/1756-0500/5/303

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http://www.biomedcentral.com/1756-0500/5/303

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