Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction

• Main Authors: Alaa Abdel-Kadhim Jawad, Alaa Hani Al-Charrakh, Hashim Raheem Tarish
• Format: Article
• Language: English
• Published: College of Veterinary Medicine 2016-06-01
• Series: Al-Qadisiyah Journal of Veterinary Medicine Sciences
• Online Access: http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/362

A total of 1200 different specimens collected from human and animals sources. 600 specimens from patients suffering from diarrhea who were admitted to Al-Diwaniya Teaching Hospital for Maternity and Children. 600 specimens from the gallbladder (bile) of cattle from Al-Diwaniya slaughterhouse. This study was conducted during the period that extended from May 2013 to April 2014. Salmonella spp. were isolated and identified using bacterial culturing on selective media, biochemical, API 20E, serotyping by polyvalent and confirmation by PCR. Polymerase chain reaction (PCR) was used to detect ompC gene encoding for biosynthesis of outer membrane protein C of Salmonella genus. The results revealed that the rate of Salmonella isolates was 0.5% (3/600) from human and 1% (6/600) from animals. The PCR technique revealed that 9 isolates of Salmonella spp. were contained ompC gene (DNA amplification showed one distinct band with molecular weight of 204 bp when electrophorized on an agarose gel). The results of this study revealed that the PCR technique had a high specificity in detection of Salmonella spp., in comparison to culture and biochemical test, Mini API 20 E and serological tests. The present study found no significant differences between human and animal isolates.