The Use of Size Exclusion Chromatography to Monitor Protein Self-Assembly

The Use of Size Exclusion Chromatography to Monitor Protein Self-Assembly

High resolution size exclusion chromatography (SEC) coupled with static light scattering (SLS) analyses were conducted to study the effect of the mobile phase ionic strength and protein concentration on the output of SEC experiments. The results highlight the effect of small changes in the mobile ph...

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Bibliographic Details
Main Authors: Alaa Adawy, Matthew R. Groves
Format: Article
Language:English
Published: MDPI AG 2017-10-01
Series:Crystals
Subjects:
size exclusion chromatography
static light scattering
protein aggregation
protein nucleation
Online Access:https://www.mdpi.com/2073-4352/7/11/331

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