Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens

Isoleucine (Ile), threonine (Thr) and tryptophan (Trp) are most likely candidates for becoming third limiting amino acids (AA) in layer diets. This work studied the effect of supplementing Ile, Thr and Trp in low-protein diets on the productive performance, egg quality and serum parameters of Hy-Li...

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Main Authors: Iván Camilo Ospina Rojas, Alice Eiko Murakami, Jamile Corina Fanhani, Karla Paola Picoli, Maria José Baptista Barbosa
Format: Article
Language:English
Published: Universidade Estadual de Londrina 2015-06-01
Series:Semina: Ciências Agrárias
Subjects:
Online Access:http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/18179
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spelling doaj-6ab816bba25f41f7be7f315edbe1b9bd2020-11-24T22:29:11ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592015-06-013631735174410.5433/1679-0359.2015v36n3p1735Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hensIván Camilo Ospina Rojas 0Alice Eiko Murakami 1Jamile Corina Fanhani 2Karla Paola Picoli 3Maria José Baptista Barbosa 4Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de Maringá Isoleucine (Ile), threonine (Thr) and tryptophan (Trp) are most likely candidates for becoming third limiting amino acids (AA) in layer diets. This work studied the effect of supplementing Ile, Thr and Trp in low-protein diets on the productive performance, egg quality and serum parameters of Hy-Line W36 laying hens. A total of 360 30-week-old laying hens were distributed in a completely randomized design into 9 treatments with 5 replications of 8 birds each. Treatments consisted of a control diet that was based on corn and soybean meal formulated with 15.5% CP. A second diet with 13% CP was formulated to meet the requirements for all essential AA of the control diet, except for Ile, Thr and Trp. The other treatments consisted of individual and combined supplementation of the aforementioned AA in the 13% CP diet. The reduction of the dietary protein level negatively influenced (p<0.05) the productivity of the hens, impairing egg production, feed conversion (kg kg-1 and kg dz-1), and egg mass. However, these variables were restored with individual and combined supplementation of the three AA in the 13% CP diet. Individually-supplemented diets with Ile, or combined with Thr and/or Trp, resulted in hens with lower (p<0.05) serum uric acid and ammonia concentrations as compared to birds receiving diets with 15.5% (control) and 13% CP. The protein levels can be reduced (13% CP) in the diet of commercial laying hens without compromising performance, egg quality or serum parameters with individual Ile, Thr or Trp supplementation. http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/18179Amino acid; Crude protein; Egg quality; Performance; Poultry
collection DOAJ
language English
format Article
sources DOAJ
author Iván Camilo Ospina Rojas
Alice Eiko Murakami
Jamile Corina Fanhani
Karla Paola Picoli
Maria José Baptista Barbosa
spellingShingle Iván Camilo Ospina Rojas
Alice Eiko Murakami
Jamile Corina Fanhani
Karla Paola Picoli
Maria José Baptista Barbosa
Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
Semina: Ciências Agrárias
Amino acid; Crude protein; Egg quality; Performance; Poultry
author_facet Iván Camilo Ospina Rojas
Alice Eiko Murakami
Jamile Corina Fanhani
Karla Paola Picoli
Maria José Baptista Barbosa
author_sort Iván Camilo Ospina Rojas
title Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
title_short Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
title_full Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
title_fullStr Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
title_full_unstemmed Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
title_sort tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens
publisher Universidade Estadual de Londrina
series Semina: Ciências Agrárias
issn 1676-546X
1679-0359
publishDate 2015-06-01
description Isoleucine (Ile), threonine (Thr) and tryptophan (Trp) are most likely candidates for becoming third limiting amino acids (AA) in layer diets. This work studied the effect of supplementing Ile, Thr and Trp in low-protein diets on the productive performance, egg quality and serum parameters of Hy-Line W36 laying hens. A total of 360 30-week-old laying hens were distributed in a completely randomized design into 9 treatments with 5 replications of 8 birds each. Treatments consisted of a control diet that was based on corn and soybean meal formulated with 15.5% CP. A second diet with 13% CP was formulated to meet the requirements for all essential AA of the control diet, except for Ile, Thr and Trp. The other treatments consisted of individual and combined supplementation of the aforementioned AA in the 13% CP diet. The reduction of the dietary protein level negatively influenced (p<0.05) the productivity of the hens, impairing egg production, feed conversion (kg kg-1 and kg dz-1), and egg mass. However, these variables were restored with individual and combined supplementation of the three AA in the 13% CP diet. Individually-supplemented diets with Ile, or combined with Thr and/or Trp, resulted in hens with lower (p<0.05) serum uric acid and ammonia concentrations as compared to birds receiving diets with 15.5% (control) and 13% CP. The protein levels can be reduced (13% CP) in the diet of commercial laying hens without compromising performance, egg quality or serum parameters with individual Ile, Thr or Trp supplementation.
topic Amino acid; Crude protein; Egg quality; Performance; Poultry
url http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/18179
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