Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays

• Main Authors: John Miller, Angelica Castaneda, Kun Ho Lee, Martin Sanchez, Adrian Ortiz, Ekrem Almaz, Zuleyha Turkoglu Almaz, Shelton Murinda, Wei-Jen Lin, Ertan Salik
• Format: Article
• Language: English
• Published: MDPI AG 2015-04-01
• Series: Biosensors
• Subjects: fiber optic biosensor; tapered fiber sensor; biosensor; immunoassay; label-free biosensor; protein detection
• Online Access: http://www.mdpi.com/2079-6374/5/2/158

We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G)-antigen (rabbit anti-mouse IgG) reactions was studied. Hydrofluoric acid treatment makes the sensitive region thinner to enhance sensitivity, which we confirmed by experiments and simulations. The limit of detection for the sensor was estimated to be less than 50 ng/mL. Utilization of the rate of the sensor peak shift within the first few minutes of the antibody-antigen reaction is proposed as a rapid protein detection method.