Natural Antioxidants Dietary and Lipid Oxidation Analysis in Zebrafish (Brachydanio rerio) Tissue

• Main Authors: ARDIANSYAH, INDRAYANI
• Format: Article
• Language: English
• Published: Bogor Agricultural University 2007-09-01
• Series: Hayati Journal of Biosciences
• Subjects: natural antioxidant; lipid oxidation; TBARS; GPx; CAT; GST
• Online Access: http://journal.ipb.ac.id/index.php/hayati/article/viewFile/245/111

Autoxidation of lipids is related to flesh deterioration and discoloration, as well as unpleasant flavors produced during processing and storage. This becomes a major factor determining fresh and frozen seafood products price. The aims of this research were to investigate the effect of two novel dietary supplementation antioxidants Vinlife® grape seed extract and Herbalox® rosemary extract on lipid oxidation during post-mortem storage of zebrafish (Brachydanio rerio) and the relationships between dietary antioxidants with the enzymatic antioxidant activities. Herbalox® supplemented diet showed no harmful effects on growth or survival of zebrafish. However, the diet could not inhibit Thiobarbituric Acid Reactive Substances (TBARS) formation in zebrafish during post-mortem storage at 4 oC. Dietary Herbalox® and Vinlife® supplementation also showed no deleterious effects on growth or survival of zebrafish. The Vinlife® supplementation efficiently suppressed TBARS formation in the zebrafish but the Herbalox® supplementation. The two novel antioxidants had significant effects on catalase and glutathione S-transferase but not on glutathione peroxidase. These findings suggested that the supplementation with Vinlife® was more effective than with Herbalox® in inhibiting the formation of lipid oxidation products in zebrafish. Hence, Vinlife® supplementation, could be a safe alternative method to improve oxidative stability of fish lipid contents.