Monitoring SARS-CoV-2 decontamination by dry heat and ultraviolet treatment with a swine coronavirus as a surrogate

• Main Authors: G. Singh, J. Jorgenson, T. Pringle, T. Nelson, S. Ramamoorthy
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: Infection Prevention in Practice
• Subjects: Decontamination; Ultraviolet; Dry heat; PEDV; SARS-CoV-2; PPE
• Online Access: http://www.sciencedirect.com/science/article/pii/S2590088920300676

Summary: The critical need for reliable methods to validate decontamination protocols for personal protective equipment (PPE) for re-use during the SARS-CoV-2 pandemic is limited by the need for specialized containment facilities to handle the virus. Hence, we have herein validated the use of a swine coronavirus as a surrogate, and tested the effectiveness of dry heat and ultraviolet (UV) rays for PPE decontamination. Exposure of experimentally contaminated N95 masks and hospital gowns to 60°C for 20 min, and UVC at 1800 mJ/cm2 resulted in a 4-log reduction and inactivation of the surrogate virus. This study provides a novel alternative to validate PPE reprocessing methods.