Study on the visible-light-induced photokilling effect of nitrogen-doped TiO<sub>2 </sub>nanoparticles on cancer cells
<p>Abstract</p> <p>Nitrogen-doped TiO<sub>2 </sub>(N-TiO<sub>2</sub>) nanoparticles were prepared by calcining the anatase TiO<sub>2 </sub>nanoparticles under ammonia atmosphere. The N-TiO<sub>2 </sub>showed higher absorbance in the v...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2011-01-01
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| Series: | Nanoscale Research Letters |
| Online Access: | http://www.nanoscalereslett.com/content/6/1/356 |
| Summary: | <p>Abstract</p> <p>Nitrogen-doped TiO<sub>2 </sub>(N-TiO<sub>2</sub>) nanoparticles were prepared by calcining the anatase TiO<sub>2 </sub>nanoparticles under ammonia atmosphere. The N-TiO<sub>2 </sub>showed higher absorbance in the visible region than the pure TiO<sub>2</sub>. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO<sub>2 </sub>were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO<sub>2 </sub>were killed more effectively than that with the pure TiO<sub>2</sub>. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO<sub>2 </sub>nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO<sub>2 </sub>nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO<sub>2</sub>-treated cells were irradiated by the visible light.</p> |
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| ISSN: | 1931-7573 1556-276X |