Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

• Main Authors: Paul L. Wood, Michelle N. Donohue, John E. Cebak, Taylor G. Beckmann, MacKenzie Treece, Jason W. Johnson, Lynda M. J. Miller
• Format: Article
• Language: English
• Published: MDPI AG 2018-11-01
• Series: Metabolites
• Subjects: bovine pink eye; infectious bovine keratoconjunctivitis (IBK), resolvin E2; cyclic phosphatidic acid; (O-acyl)-ω-hydroxy-fatty acids; plasmalogens; sphingomyelins; ocular inflammation; tear film fluid
• Online Access: https://www.mdpi.com/2218-1989/8/4/81

<i>Background</i>: Tear film fluid serves as a dynamic barrier that both lubricates the eye and protects against allergens and infectious agents. However, a detailed analysis of a bacteria-induced immune response on the tear film lipidome has not been undertaken. <i>Methods</i>: We undertook a high-resolution mass spectrometry lipidomics analysis of endogenous anti-inflammatory and structural tear film lipids in bovine pink eye. <i>Results</i>: Bovine pink eye resulted in dramatic elevations in tear fluid levels of the anti-inflammatory lipids resolvin E2, cyclic phosphatidic acid 16:0, and cyclic phosphatidic acid 18:0. In addition, there were elevated levels of the structural lipids (O-acyl)-&#969;-hydroxy-fatty acids, cholesterol sulfate, ethanolamine plasmalogens, and sphingomyelins. Lipid peroxidation also was augmented in pink eye as evidenced by the hydroperoxy derivatives of ethanolamine plasmalogens. <i>Conclusions</i>: Ocular infections with <i>Moraxella bovis</i> result in the induction of a number of endogenous anti-inflammatory lipids and augmentation of the levels of structural glycerophospholipids and sphingolipids. Increased levels of hydroperoxy glycerophospholipids also indicate that this bacterial infection results in lipid peroxidation.