Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein inte...

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Main Authors: Mi Ke, Xiao Yuan, An He, Peiyuan Yu, Wendong Chen, Yu Shi, Tony Hunter, Peng Zou, Ruijun Tian
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20367-x
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spelling doaj-6a106013d625450083626aaeed6b1d452021-01-10T12:19:42ZengNature Publishing GroupNature Communications2041-17232021-01-0112111310.1038/s41467-020-20367-xSpatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomicsMi Ke0Xiao Yuan1An He2Peiyuan Yu3Wendong Chen4Yu Shi5Tony Hunter6Peng Zou7Ruijun Tian8Department of Chemistry, School of Science, Southern University of Science and TechnologyDepartment of Chemistry, School of Science, Southern University of Science and TechnologyDepartment of Chemistry, School of Science, Southern University of Science and TechnologyDepartment of Chemistry, School of Science, Southern University of Science and TechnologyDepartment of Chemistry, School of Science, Southern University of Science and TechnologyMolecular and Cell Biology Laboratory, Salk Institute for Biological StudiesMolecular and Cell Biology Laboratory, Salk Institute for Biological StudiesCollege of Chemistry and Molecular Engineering, Peking UniversityDepartment of Chemistry, School of Science, Southern University of Science and TechnologyAPEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein interactomes.https://doi.org/10.1038/s41467-020-20367-x
collection DOAJ
language English
format Article
sources DOAJ
author Mi Ke
Xiao Yuan
An He
Peiyuan Yu
Wendong Chen
Yu Shi
Tony Hunter
Peng Zou
Ruijun Tian
spellingShingle Mi Ke
Xiao Yuan
An He
Peiyuan Yu
Wendong Chen
Yu Shi
Tony Hunter
Peng Zou
Ruijun Tian
Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
Nature Communications
author_facet Mi Ke
Xiao Yuan
An He
Peiyuan Yu
Wendong Chen
Yu Shi
Tony Hunter
Peng Zou
Ruijun Tian
author_sort Mi Ke
title Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_short Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_full Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_fullStr Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_full_unstemmed Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_sort spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-01-01
description APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein interactomes.
url https://doi.org/10.1038/s41467-020-20367-x
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AT xiaoyuan spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT anhe spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT peiyuanyu spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT wendongchen spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT yushi spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT tonyhunter spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT pengzou spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
AT ruijuntian spatiotemporalprofilingofcytosolicsignalingcomplexesinlivingcellsbyselectiveproximityproteomics
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