Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate

The investigated integrated bioprocess for the production of bioethanol from lignocellulosic biomass, derived from the perennial herbaceous species Phalaris aquatica L., consists of three main stages, which were studied and optimised separately. The pretreatment process was investigated through a Ta...

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Main Authors: A. Karapatsia, G. Penloglou, I. Pappas, C. Kiparissides
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2014-06-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/5743
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spelling doaj-69fa6589445d451996c43380cfef329c2021-02-21T21:00:51ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162014-06-013710.3303/CET1437049Bioethanol Production via the Fermentation of Phalaris aquatica L. HydrolysateA. KarapatsiaG. PenloglouI. PappasC. KiparissidesThe investigated integrated bioprocess for the production of bioethanol from lignocellulosic biomass, derived from the perennial herbaceous species Phalaris aquatica L., consists of three main stages, which were studied and optimised separately. The pretreatment process was investigated through a Taguchi statistical design where mild conditions were selected for the optimization of the hemicellulose hydrolysis. The solid residue, where cellulose lies, was enzymatically hydrolysed with the implementation of a Box-Denken statistical design and glucose was recovered at a concentration equal to 13.5 g/L. Glucose concentration increased to 45 g/L after the application of a semi-batch policy. The fermentation of the enzymatic hydrolysate with the yeast Saccharomyces cerevisiae was initially conducted under batch conditions in flask scale and the optimal regime was selected to be transferred to the bioreactor scale. Inoculum size, aeration, agitation speed, fermentation medium composition and type of strain were the parameters of study. The fermentation of the hydrolysate provided 47 g/L ethanol concentration under fed-batch conditions, corresponding to 90 % yield. The specific productivity was equal to 4.8 g/(L·h), while the overall productivity was maximized, through different feeding policies, to 2.3 g/(L·h).https://www.cetjournal.it/index.php/cet/article/view/5743
collection DOAJ
language English
format Article
sources DOAJ
author A. Karapatsia
G. Penloglou
I. Pappas
C. Kiparissides
spellingShingle A. Karapatsia
G. Penloglou
I. Pappas
C. Kiparissides
Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
Chemical Engineering Transactions
author_facet A. Karapatsia
G. Penloglou
I. Pappas
C. Kiparissides
author_sort A. Karapatsia
title Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
title_short Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
title_full Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
title_fullStr Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
title_full_unstemmed Bioethanol Production via the Fermentation of Phalaris aquatica L. Hydrolysate
title_sort bioethanol production via the fermentation of phalaris aquatica l. hydrolysate
publisher AIDIC Servizi S.r.l.
series Chemical Engineering Transactions
issn 2283-9216
publishDate 2014-06-01
description The investigated integrated bioprocess for the production of bioethanol from lignocellulosic biomass, derived from the perennial herbaceous species Phalaris aquatica L., consists of three main stages, which were studied and optimised separately. The pretreatment process was investigated through a Taguchi statistical design where mild conditions were selected for the optimization of the hemicellulose hydrolysis. The solid residue, where cellulose lies, was enzymatically hydrolysed with the implementation of a Box-Denken statistical design and glucose was recovered at a concentration equal to 13.5 g/L. Glucose concentration increased to 45 g/L after the application of a semi-batch policy. The fermentation of the enzymatic hydrolysate with the yeast Saccharomyces cerevisiae was initially conducted under batch conditions in flask scale and the optimal regime was selected to be transferred to the bioreactor scale. Inoculum size, aeration, agitation speed, fermentation medium composition and type of strain were the parameters of study. The fermentation of the hydrolysate provided 47 g/L ethanol concentration under fed-batch conditions, corresponding to 90 % yield. The specific productivity was equal to 4.8 g/(L·h), while the overall productivity was maximized, through different feeding policies, to 2.3 g/(L·h).
url https://www.cetjournal.it/index.php/cet/article/view/5743
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