Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase

A biosensor for rapid determination of nonsteroidal anti-inflammatory drugs (NSAIDs) is described based on the inhibition of cyclooxygenase enzyme (both isoforms) by NSAIDs. The results show the full validity of the method, which has also been optimized by comparing the inhibition of two enzyme isof...

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Main Authors:	Giulia Di Persio, Manuela Pintore, Daniele Tonnina, Nicola Caretto, Elisabetta Martini, Dalina Lelo, Luigi Campanella
Format:	Article
Language:	English
Published:	University of Zagreb 2009-01-01
Series:	Food Technology and Biotechnology
Subjects:	food analysis COX inhibition biosensor
Online Access:	http://hrcak.srce.hr/file/59879

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spelling	doaj-69f63ef09e174371ab65eb225b41cdb62020-11-25T00:41:02ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062009-01-01472172177Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of CyclooxygenaseGiulia Di Persio0Manuela Pintore1Daniele Tonnina2Nicola Caretto3Elisabetta Martini4Dalina Lelo5Luigi Campanella6Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyDepartment of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, ItalyA biosensor for rapid determination of nonsteroidal anti-inflammatory drugs (NSAIDs) is described based on the inhibition of cyclooxygenase enzyme (both isoforms) by NSAIDs. The results show the full validity of the method, which has also been optimized by comparing the inhibition of two enzyme isoforms, COX-1 and COX-2, in the presence of different tested pharmaceutical drugs (diclofenac, naproxen, ibuprofen, tolmetin). Also, recovery trials were performed in milk and fresh cheese adulterated with known quantities of NSAIDs, always obtaining recovery values >88 %.http://hrcak.srce.hr/file/59879food analysisCOXinhibition biosensor
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Giulia Di Persio Manuela Pintore Daniele Tonnina Nicola Caretto Elisabetta Martini Dalina Lelo Luigi Campanella
spellingShingle	Giulia Di Persio Manuela Pintore Daniele Tonnina Nicola Caretto Elisabetta Martini Dalina Lelo Luigi Campanella Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase Food Technology and Biotechnology food analysis COX inhibition biosensor
author_facet	Giulia Di Persio Manuela Pintore Daniele Tonnina Nicola Caretto Elisabetta Martini Dalina Lelo Luigi Campanella
author_sort	Giulia Di Persio
title	Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase
title_short	Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase
title_full	Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase
title_fullStr	Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase
title_full_unstemmed	Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase
title_sort	determination of nonsteroidal anti-inflammatory drugs (nsaids) in milk and fresh cheese based on the inhibition of cyclooxygenase
publisher	University of Zagreb
series	Food Technology and Biotechnology
issn	1330-9862 1334-2606
publishDate	2009-01-01
description	A biosensor for rapid determination of nonsteroidal anti-inflammatory drugs (NSAIDs) is described based on the inhibition of cyclooxygenase enzyme (both isoforms) by NSAIDs. The results show the full validity of the method, which has also been optimized by comparing the inhibition of two enzyme isoforms, COX-1 and COX-2, in the presence of different tested pharmaceutical drugs (diclofenac, naproxen, ibuprofen, tolmetin). Also, recovery trials were performed in milk and fresh cheese adulterated with known quantities of NSAIDs, always obtaining recovery values >88 %.
topic	food analysis COX inhibition biosensor
url	http://hrcak.srce.hr/file/59879
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Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase

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