Scaffold architecture and fibrin gels promote meniscal cell proliferation

Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotro...

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Main Authors:	K. M. Pawelec, S. M. Best, R. E. Cameron, R. J. Wardale
Format:	Article
Language:	English
Published:	AIP Publishing LLC 2015-01-01
Series:	APL Materials
Online Access:	http://dx.doi.org/10.1063/1.4900885

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spelling	doaj-69651cf5d1b64dffa380d2d15c6f60ad2020-11-25T02:26:20ZengAIP Publishing LLCAPL Materials2166-532X2015-01-0131014901014901-710.1063/1.4900885001595APMScaffold architecture and fibrin gels promote meniscal cell proliferationK. M. Pawelec0S. M. Best1R. E. Cameron2R. J. Wardale3Cambridge Centre for Medical Materials, Materials Science and Metallurgy Department, University of Cambridge, Cambridge CB3 0FS, United KingdomCambridge Centre for Medical Materials, Materials Science and Metallurgy Department, University of Cambridge, Cambridge CB3 0FS, United KingdomCambridge Centre for Medical Materials, Materials Science and Metallurgy Department, University of Cambridge, Cambridge CB3 0FS, United KingdomDivision of Trauma and Orthopaedic Surgery, Department of Surgery, University of Cambridge, Cambridge CB2 2QQ, United Kingdom Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation. http://dx.doi.org/10.1063/1.4900885
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	K. M. Pawelec S. M. Best R. E. Cameron R. J. Wardale
spellingShingle	K. M. Pawelec S. M. Best R. E. Cameron R. J. Wardale Scaffold architecture and fibrin gels promote meniscal cell proliferation APL Materials
author_facet	K. M. Pawelec S. M. Best R. E. Cameron R. J. Wardale
author_sort	K. M. Pawelec
title	Scaffold architecture and fibrin gels promote meniscal cell proliferation
title_short	Scaffold architecture and fibrin gels promote meniscal cell proliferation
title_full	Scaffold architecture and fibrin gels promote meniscal cell proliferation
title_fullStr	Scaffold architecture and fibrin gels promote meniscal cell proliferation
title_full_unstemmed	Scaffold architecture and fibrin gels promote meniscal cell proliferation
title_sort	scaffold architecture and fibrin gels promote meniscal cell proliferation
publisher	AIP Publishing LLC
series	APL Materials
issn	2166-532X
publishDate	2015-01-01
description	Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation.
url	http://dx.doi.org/10.1063/1.4900885
work_keys_str_mv	AT kmpawelec scaffoldarchitectureandfibringelspromotemeniscalcellproliferation AT smbest scaffoldarchitectureandfibringelspromotemeniscalcellproliferation AT recameron scaffoldarchitectureandfibringelspromotemeniscalcellproliferation AT rjwardale scaffoldarchitectureandfibringelspromotemeniscalcellproliferation
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Scaffold architecture and fibrin gels promote meniscal cell proliferation

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