Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.

Bibliographic Details
Main Authors: Mark Pogson, Cristina Parola, William J. Kelton, Paul Heuberger, Sai T. Reddy
Format: Article
Language:English
Published: Nature Publishing Group 2016-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms12535
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spelling doaj-6925af93eca14d3ca5ad753540c1ac3f2021-05-11T11:07:57ZengNature Publishing GroupNature Communications2041-17232016-08-017111010.1038/ncomms12535Immunogenomic engineering of a plug-and-(dis)play hybridoma platformMark Pogson0Cristina Parola1William J. Kelton2Paul Heuberger3Sai T. Reddy4Department of Biosystems Science and Engineering, ETH ZurichDepartment of Biosystems Science and Engineering, ETH ZurichDepartment of Biosystems Science and Engineering, ETH ZurichDepartment of Biosystems Science and Engineering, ETH ZurichDepartment of Biosystems Science and Engineering, ETH ZurichHybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.https://doi.org/10.1038/ncomms12535
collection DOAJ
language English
format Article
sources DOAJ
author Mark Pogson
Cristina Parola
William J. Kelton
Paul Heuberger
Sai T. Reddy
spellingShingle Mark Pogson
Cristina Parola
William J. Kelton
Paul Heuberger
Sai T. Reddy
Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
Nature Communications
author_facet Mark Pogson
Cristina Parola
William J. Kelton
Paul Heuberger
Sai T. Reddy
author_sort Mark Pogson
title Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
title_short Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
title_full Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
title_fullStr Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
title_full_unstemmed Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
title_sort immunogenomic engineering of a plug-and-(dis)play hybridoma platform
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2016-08-01
description Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
url https://doi.org/10.1038/ncomms12535
work_keys_str_mv AT markpogson immunogenomicengineeringofapluganddisplayhybridomaplatform
AT cristinaparola immunogenomicengineeringofapluganddisplayhybridomaplatform
AT williamjkelton immunogenomicengineeringofapluganddisplayhybridomaplatform
AT paulheuberger immunogenomicengineeringofapluganddisplayhybridomaplatform
AT saitreddy immunogenomicengineeringofapluganddisplayhybridomaplatform
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