Variations in the viability and macromolecules concentration of E. granulosus protoscolices isolated from ruminants consequence treatment with Nigella sativa seed's oil (In vitro study)

• Main Author: Maimonah Q. Yahya
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2019-09-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: nigella sativa; e. granulosus; seeds oil; protoscolices; biomolecules
• Online Access: https://vetmedmosul.com/article_162880_c8314a5ca5dad8454e15175ca08a8b61.pdf

The aim of the present study was to investigate the scolicidal activity of <em>Nigella sativa</em> seed<strong>'</strong>s oil concerning the viability <em>in vitro</em> and biomolecules content (carbohydrates, proteins and nucleic acids) of<em>Echinococcus granulosus</em> protoscolices. Protoscolices were aseptically aspiratedfromcysts of livers and lungs (naturally infected) which eradicated from goats and sheep that had been slaughtered at Mosul local abattoir / Nineveh / Iraq from September 2017 to May 2018. Various concentrations of <em>N. sativa</em> seed's oil (20 to 60 µl/ml) were applied on special time interval (5-40min). Viability of protoscolices was checked using vital stain (0.1% aqueous eosin). Concentration of carbohydrates, proteins and nucleic acids (DNA and RNA) were estimated after treating the protoscolices with LC₅₀ of the seed's oil (40 µl/ml) comparing with untreated group. It is revealed that mortality rate of protoscolices, those were treated for 10 minutes with 40 µl/ml and 60 µl/ml of <em>Nigella sativa</em> oil, were approximately 50% and 100% respectively. Viability % of protoscolices treated with 20% hypertonic saline solution for 5 minutes was 43%, whereas, 16% of protoscolices were viable when treated with 60 µl/ml of seed's oilat the same time. The experiments detect concentration and time-dependent scolicidal effect of <em>N. sativa </em>seed's oilon the <em>E. granulosus</em> protoscolices. Mean concentrations of carbohydrates, proteins and nucleic acids (DNA and RNA) were significantly higher at P≤ 0.05 in control group (62.6µg/ml, 31.0 mg/dl and (23.4 and 82.9 µg/ml, respectively) than that found in LC₅₀ treated protoscolices (58.3 µg/ml, 15.3 mg/dl and (18.19 and 64.48 µg/ml, respectively).The study showed that oil extract of <em>N. sativa </em>seedshas a significant (P≤ 0.05) clear impact in reducing viability of <em>E. granulosus </em>protoscolices, as well as, mean concentrations of its biomolecules which may open away for further experiments about scolicidal validity of <em>N. sativa</em> seeds oil <em>in vivo</em>.