The contribution of S100B to the glioprotective effects of valproic and arundic acids

• Main Authors: Mojtaba Keshavarz, Majid Reza Farrokhi, Atena Amiri, Khadijeh Hosseini
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2019-05-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Amyloid-β-peptide; Arundic acid; Astrocytes; S100B; Valproic acid
• Online Access: http://ijbms.mums.ac.ir/article_12273_7c3d43dd17bc2f86ea049146ee7d4baf.pdf
• ISSN: 2008-3866; 2008-3874

Objective(s): Valproic and arundic acids are astrocytes-modulating agents with potential effects in the treatment of Alzheimer’s disease (AD). S100B is an astrocytic cytokine with a possible role in the pathogenesis of AD. In this study, we aimed to assess the glioprotective effects of valproic and arundic acids against amyloid-β-peptide (Aβ)-induced glial death and contribution of S100B to the glioprotective effects of these agents in an astrocytic culture.   Materials and Methods: We used Aβ25–35 at a concentration of 200 μM in 1321N1 astrocyte cells. We treated the cells with valproic acid (0.5 and 1 mM) and/or arundic acid 50 µM for 24 hr. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) test was used to measure cell viability. The intracellular and extracellular S100B levels were measured using an ELISA kit. The data were analyzed using one-way analysis of variance followed by the Tukey’s test.   Results: Aβ (200 µM) decreased the cell viability compared to the control group (P