Development of golden hamster embryos effectively produced by injection of sperm heads sonicated in Tris‐HCl buffer with EGTA
Abstract Purpose To investigate the effects of sperm treatment medium—TCM199 or EGTA in Tris‐HCl buffer (TBS + EGTA)―for sonication of frozen‐thawed hamster spermatozoa in terms of sperm chromosome integrity and development of hamster oocytes injected with the sperm heads (ICSI). Methods Frozen‐thaw...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
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| Series: | Reproductive Medicine and Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/rmb2.12253 |