CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

Summary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent t...

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Bibliographic Details
Main Authors: Caroline Hoppe, Hilary L. Ashe
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Genetics
Model organisms
Molecular biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000873
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spelling doaj-68c80ef47072438c825250851d9335732021-03-22T12:53:33ZengElsevierSTAR Protocols2666-16672021-03-0121100380CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryosCaroline Hoppe0Hilary L. Ashe1Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK; Corresponding authorFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK; Corresponding authorSummary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila.For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000873GeneticsModel organismsMolecular biologyCRISPR
collection DOAJ
language English
format Article
sources DOAJ
author Caroline Hoppe
Hilary L. Ashe
spellingShingle Caroline Hoppe
Hilary L. Ashe
CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
STAR Protocols
Genetics
Model organisms
Molecular biology
CRISPR
author_facet Caroline Hoppe
Hilary L. Ashe
author_sort Caroline Hoppe
title CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_short CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_full CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_fullStr CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_full_unstemmed CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_sort crispr-cas9 strategies to insert ms2 stem-loops into endogenous loci in drosophila embryos
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila.For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).
topic Genetics
Model organisms
Molecular biology
CRISPR
url http://www.sciencedirect.com/science/article/pii/S2666166721000873
work_keys_str_mv AT carolinehoppe crisprcas9strategiestoinsertms2stemloopsintoendogenouslociindrosophilaembryos
AT hilarylashe crisprcas9strategiestoinsertms2stemloopsintoendogenouslociindrosophilaembryos
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