CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

Summary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent t...

Full description

Bibliographic Details
Main Authors: Caroline Hoppe, Hilary L. Ashe
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000873
Description
Summary:Summary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila.For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).
ISSN:2666-1667