Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using ant...
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doaj-68a34e64727848539317b920a004ed272020-11-25T00:20:32ZengBMCBMC Microbiology1471-21802007-05-01713810.1186/1471-2180-7-38Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membraneLiu KaiyangJia TianjunZhong YouminLiu GuangchaoLuo JianhuaChen DingZhong Guangming<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p> http://www.biomedcentral.com/1471-2180/7/38 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming |
spellingShingle |
Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane BMC Microbiology |
author_facet |
Liu Kaiyang Jia Tianjun Zhong Youmin Liu Guangchao Luo Jianhua Chen Ding Zhong Guangming |
author_sort |
Liu Kaiyang |
title |
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
title_short |
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
title_full |
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
title_fullStr |
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
title_full_unstemmed |
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane |
title_sort |
characterization of hypothetical proteins cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>chlamydia pneumoniae </it>inclusion membrane |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2007-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p> |
url |
http://www.biomedcentral.com/1471-2180/7/38 |
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