Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane

<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using ant...

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Main Authors: Liu Kaiyang, Jia Tianjun, Zhong Youmin, Liu Guangchao, Luo Jianhua, Chen Ding, Zhong Guangming
Format: Article
Language:English
Published: BMC 2007-05-01
Series:BMC Microbiology
Online Access:http://www.biomedcentral.com/1471-2180/7/38
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spelling doaj-68a34e64727848539317b920a004ed272020-11-25T00:20:32ZengBMCBMC Microbiology1471-21802007-05-01713810.1186/1471-2180-7-38Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membraneLiu KaiyangJia TianjunZhong YouminLiu GuangchaoLuo JianhuaChen DingZhong Guangming<p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p> http://www.biomedcentral.com/1471-2180/7/38
collection DOAJ
language English
format Article
sources DOAJ
author Liu Kaiyang
Jia Tianjun
Zhong Youmin
Liu Guangchao
Luo Jianhua
Chen Ding
Zhong Guangming
spellingShingle Liu Kaiyang
Jia Tianjun
Zhong Youmin
Liu Guangchao
Luo Jianhua
Chen Ding
Zhong Guangming
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
BMC Microbiology
author_facet Liu Kaiyang
Jia Tianjun
Zhong Youmin
Liu Guangchao
Luo Jianhua
Chen Ding
Zhong Guangming
author_sort Liu Kaiyang
title Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
title_short Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
title_full Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
title_fullStr Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
title_full_unstemmed Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>Chlamydia pneumoniae </it>inclusion membrane
title_sort characterization of hypothetical proteins cpn0146, 0147, 0284 & 0285 that are predicted to be in the <it>chlamydia pneumoniae </it>inclusion membrane
publisher BMC
series BMC Microbiology
issn 1471-2180
publishDate 2007-05-01
description <p>Abstract</p> <p>Background</p> <p>Although more than 100 <it>Chlamydia pneumoniae </it>hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<it>Cpn0146-147 </it>and <it>Cpn0284-285</it>) in the <it>C. pneumoniae </it>genome.</p> <p>Results</p> <p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with <it>C. pneumoniae </it>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the <it>C. pneumoniae </it>inclusion membrane proteins did not result in inhibition of the subsequent <it>C. pneumoniae </it>infection.</p> <p>Conclusion</p> <p>The hypothetical proteins Cpn0146 & 0147 were localized in the <it>C. pneumoniae </it>inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.</p>
url http://www.biomedcentral.com/1471-2180/7/38
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