Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals

Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals

Flavin-dependent halogenases chlorinate or brominate their substrates in an environmentally friendly manner, only requiring the cofactor reduced flavin adenine dinucleotide (FADH<sub>2</sub>), oxygen, and halide salts. The tryptophan 6-halogenase Thal exhibits two flexible loops, which b...

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Bibliographic Details
Main Authors: Ann-Christin Moritzer, Tina Prior, Hartmut H. Niemann
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Crystals
Subjects:
flavin-dependent halogenase
protein crystallization
tryptophan halogenase
ThdH
Online Access:https://www.mdpi.com/2073-4352/10/12/1135

Internet

https://www.mdpi.com/2073-4352/10/12/1135

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