The Correlation between OCT4B1 and Apoptosis in Human Cancer Cell Lines
ABSTRACT BACKGROUND AND OBJECTIVE: OCT4B1, as one of the variants of OCT4 gene, is expressed at higher levels in cancer tissues and cancer cell lines, compared to other OCT4 variants. Recent studies have revealed the anti-apoptotic role of OCT4B1. The aim of this study was to evaluate the effect of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Babol University of Medical Sciences
2015-01-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| Subjects: | |
| Online Access: | http://jbums.org/browse.php?a_code=A-10-1552-1&slc_lang=en&sid=1 |
| Summary: | ABSTRACT
BACKGROUND AND
OBJECTIVE: OCT4B1, as one of the variants of OCT4 gene, is expressed
at higher levels in cancer tissues and cancer cell lines, compared to other
OCT4 variants. Recent studies have revealed the anti-apoptotic role of OCT4B1.
The aim of this study was to evaluate the effect of OCT4B1 suppression on the
expression profile of anti-apoptotic genes in three human tumor cell lines.
METHODS: In
this in-vitro study, three human tumor cell lines including AGS (stomach
adenocarcinoma), 5637 (bladder cancer), and U87MG (brain tumor) were purchased
from the National Cell Bank of Iran (Pasteur Institute) and cultured in test
and control groups. In order to suppress OCT4B1 expression, the cultures were transfected,
using siRNA technology and lipofection method. After confirming the suppression
process, total RNA was extracted and cDNA was synthesized. Finally, the
expression rates of anti-apoptotic genes were determined, using specific
primers and real-time PCR technique.
FINDINGS: Our data revealed an almost similar
pattern of alteration in the expression profile of anti-apoptotic genes in all
three cell lines. Also, BCL2, BRAF, and BFAR genes exhibited the most
significant down-regulation by 20.87, 18.33, and 15.11 folds, respectively. The
expression of at least 20 genes (out of 26 genes) decreased, while the
expression rates of CASP2, IGF1R, TNF, and MCL1 were up-regulated or remained
unchanged. Also, the expression of CFLAR gene was up-regulated in U87MG and
down-regulated in other cell lines (5637 and AGS).
CONCLUSION: Based
on our findings, OCT4B1 suppression by blocking the expression of
anti-apoptotic genes may result in the induction of apoptosis in cancer cell
lines. |
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| ISSN: | 1561-4107 2251-7170 |