Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing

Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing

<p>Abstract</p> <p>Background</p> <p>In addition to shotgun sequencing, next generation sequencing has been shown to be suitable for deep sequencing of many specific PCR-amplified target genes in parallel. However, unspecific product formation is a common problem in amp...

Full description

Bibliographic Details
Main Authors:	Ahmadian Afshin, Neiman Marten, Sandberg Julia, Lundeberg Joakim
Format:	Article
Language:	English
Published:	BMC 2010-02-01
Series:	BMC Genomics
Online Access:	http://www.biomedcentral.com/1471-2164/11/140

Similar Items

Sorting Out the FACS: A Devil in the Details
by: William C. Hines, et al.
Published: (2014-03-01)

AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities.
by: Anja Lange, et al.
Published: (2015-01-01)

The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing
by: Horatio H. Morgan, et al.
Published: (2017-05-01)

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing
by: Federico Mussano, et al.
Published: (2018-04-01)

Apical periodontitis: Preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing
by: Carossa, S., et al.
Published: (2018)

Large scale library generation for high throughput sequencing.
by: Erik Borgström, et al.
Published: (2011-01-01)

Decoding a substantial set of samples in parallel by massive sequencing.
by: Mårten Neiman, et al.
Published: (2011-01-01)

The use of high-throughput amplicon deep sequencing to explore aquatic virus communities
by: Tian, Xi
Published: (2015)

Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)
by: Benyamin Rosental, et al.
Published: (2017-08-01)

Computational Analysis of AmpSeq Data for Targeted, High-Throughput Genotyping of Amplicons
by: Jonathan Fresnedo-Ramírez, et al.
Published: (2019-05-01)

High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
by: Benjamin Strobel, et al.
Published: (2020-02-01)

Application of high-throughput amplicon sequencing-based SSR genotyping in genetic background screening
by: Tiantian Li, et al.
Published: (2019-06-01)

A priori Considerations When Conducting High-Throughput Amplicon-Based Sequence Analysis
by: Aditi Sengupta, et al.
Published: (2016-03-01)

Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing.
by: Ulrike Menzel, et al.
Published: (2014-01-01)

Two-stage clustering (TSC): a pipeline for selecting operational taxonomic units for the high-throughput sequencing of PCR amplicons.
by: Xiao-Tao Jiang, et al.
Published: (2012-01-01)

FACS: a high throughput method for protein export and engineering
by: Ribnicky, Brian Michael
Published: (2008)

Increased throughput by parallelization of library preparation for massive sequencing.
by: Sverker Lundin, et al.
Published: (2010-01-01)

Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay
by: Fei Li, et al.
Published: (2021-06-01)

The Microbiota of Edam Cheeses Determined by Cultivation and High-Throughput Sequencing of the 16S rRNA Amplicon
by: Beata Nalepa, et al.
Published: (2020-06-01)

PVAmpliconFinder: a workflow for the identification of human papillomaviruses from high-throughput amplicon sequencing
by: Alexis Robitaille, et al.
Published: (2020-06-01)

The effect of reverse transcription enzymes and conditions on high throughput amplicon sequencing of the 16S rRNA
by: Adam Šťovíček, et al.
Published: (2019-10-01)

Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons
by: Siddiqui Huma, et al.
Published: (2011-11-01)

Bacterial Contamination of Plant in vitro Cultures in Commercial Production Detected by High‑Throughput Amplicon Sequencing
by: Dorota Tekielska, et al.
Published: (2019-01-01)

Diversity of Endophytic Fungal Community in Leaves of Artemisia argyi Based on High-throughput Amplicon Sequencing
by: QIU-FANG WU, et al.
Published: (2021-05-01)

Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing
by: Yoshihiko Onda, et al.
Published: (2018-03-01)

Targeted RNA-sequencing with competitive multiplex-PCR amplicon libraries.
by: Thomas M Blomquist, et al.
Published: (2013-01-01)

Analysis and Visualization Tool for Targeted Amplicon Bisulfite Sequencing on Ion Torrent Sequencers.
by: Stephan Pabinger, et al.
Published: (2016-01-01)

Unraveling Core Functional Microbiota in Traditional Solid-State Fermentation by High-Throughput Amplicons and Metatranscriptomics Sequencing
by: Zhewei Song, et al.
Published: (2017-07-01)

Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
by: Thomas M. Blomquist, et al.
Published: (2013-01-01)

Optimisation and Benchmarking of Targeted Amplicon Sequencing for Mycobiome Analysis of Respiratory Specimens
by: Nur A’tikah Binte Mohamed Ali, et al.
Published: (2019-10-01)

DUDE-Seq: Fast, flexible, and robust denoising for targeted amplicon sequencing.
by: Byunghan Lee, et al.
Published: (2017-01-01)

Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries.
by: Thomas M. Blomquist, et al.
Published: (2013-01-01)

Tagging systems for sequencing large cohorts
by: Neiman, Mårten
Published: (2010)

High-Throughput Sequencing and Unsupervised Analysis of Formyltetrahydrofolate Synthetase (FTHFS) Gene Amplicons to Estimate Acetogenic Community Structure
by: Abhijeet Singh, et al.
Published: (2020-08-01)

Complementary Use of Cultivation and High-Throughput Amplicon Sequencing Reveals High Biodiversity Within Raw Milk Microbiota
by: Franziska Breitenwieser, et al.
Published: (2020-07-01)

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform
by: Johanna B. Holm, et al.
Published: (2019-02-01)

Characterizing partial AZFc deletions of the Y chromosome with amplicon-specific sequence markers
by: Nunes Joaquim, et al.
Published: (2007-09-01)

Unequivocal identification of subpopulations in putative multiclonal Trypanosoma cruzi strains by FACs single cell sorting and genotyping.
by: Helder Magno Silva Valadares, et al.
Published: (2012-01-01)

A high-throughput amplicon-based method for estimating outcrossing rates
by: Friederike Jantzen, et al.
Published: (2019-05-01)

Ultra-High-Throughput Single-Cell Functional Screening by Combination of Microfluidics and GMD-FACS
by: Yanakieva, Desislava
Published: (2020)

Cannot write session to /tmp/vufind_sessions/sess_lnmm7ocq2domms5kqq27a5pjah