Intraosseous Injection of Calcium Phosphate Polymer-Induced Liquid Precursor Increases Bone Density and Improves Early Implant Osseointegration in Ovariectomized Rats

• Main Authors: Zhou Y, Hu Z, Ge M, Jin W, Tang R, Li Q, Xu W, Shi J, Xie Z
• Format: Article
• Language: English
• Published: Dove Medical Press 2021-09-01
• Series: International Journal of Nanomedicine
• Subjects: polymer induced liquid-precursor; calcium phosphates; nanocluster; osteoporosis; oral implant
• Online Access: https://www.dovepress.com/intraosseous-injection-of-calcium-phosphate-polymer-induced-liquid-pre-peer-reviewed-fulltext-article-IJN

Yanyan Zhou,1 Zihe Hu,1 Mingjie Ge,1 Wenjing Jin,1 Ruikang Tang,2 Qi Li,1 Weijian Xu,1 Jue Shi,1 Zhijian Xie1 1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, People’s Republic of China; 2Center for Biomaterials and Biopathways, Department of Chemistry, Zhejiang University, Hangzhou, 310027, People’s Republic of ChinaCorrespondence: Zhijian Xie; Jue Shi Email [e-mail]; [e-mail]: Osteoporosis, due to bone loss and structural deterioration, is a risk factor for dental implant failure, as it impedes initial stability and osseointegration. We aim to assess the effects of calcium phosphate polymer-induced liquid precursor (CaP-PILP) treatment, which significantly increases bone density and improves early implant osseointegration in ovariectomized rats.Methods: In this study, CaP-PILP was synthesized and characterized through TEM, FTIR and XRD. A rat model of osteoporosis was generated by ovariectomy. CaP-PILP or hydroxyapatite (HAP, negative control) was injected into the tibia, and the resulting changes in bone quality were determined. Further, implants were installed in the treated tibias, and implantation characteristics were assessed after 4 weeks.Results: The CaP-PILP group had superior bone repair. Importantly, CaP-PILP had excellent properties, similar to those of normal bone, in terms of implant osseointegration. In vivo experiment displayed that CaP-PILP group had better bone contact rate (65.97± 3.176) than HAP and OVX groups. Meanwhile, a mound of mature and continuous new bone formed. Moreover, the values of BIC and BA showed no significant difference between the CaP-PILP group and the sham group.Conclusion: In summary, CaP-PILP is a promising material for application in poor-quality bones to improve implant success rates in patients with osteoporosis. This research provides new perspectives on the application of nano-apatite materials in bone repair.Keywords: polymer-induced liquid precursor, calcium phosphates, nanocluster, osteoporosis, oral implant